iSTAX® Plate Stacking Solutions

Plate Stackers

iSTAX are modular plate stacker storage devices for microplates and nested tip racks. When coupled to the Linear Rail Transport System, or LRTS(r), iSTAX provide an ideal solution for extended walk-away lab automation.

Each stack is capable of holding up to 40 SBS standard micro plates. Built with industrial grade components, iSTAX technology allows plates or lids to be quickly loaded or unloaded from the LRTS. iSTAX includes an Instrument Control Program (ICP) that allows users to configure and exercise individual stacks, or groups of stacks. Stacks may be set-up to process plates individually or in groups, providing exceptional flexibility and high throughput. iSTAX racks are compatible with Caliper's Twister II plate handling laboratory robotics systems, allowing for easy transport to assay systems and snap in loading.

IVIS® Kinetic

The IVIS Kinetic from Caliper Life Sciences provides a real time, fast imaging system enabling acquisition of biologically relevant events within milliseconds. The IVIS Kinetic can perform both quantitative luminescence and fluorescence as a standard high signal to noise imager and as a high speed imager. The system includes a highly sensitive EMCCD camera for signal enhancement and the ability to reduce exposure times enabling fast kinetics. From the leading optical imaging platform for in vivo analysis, IVIS systems are supported by a range of practical accessories developed through experience in research laboratories worldwide. The IVIS Kinetic offers a light-tight injection port which supporting a syringe injector system enabling real time compound and/or substrate administration. A removable pull-out tray is available to facilitate pre-imaging animal procedures.

or contact David Panzarella at +1-845-735-1921 or David.Panzarella@perkinelmer.com.

IVIS® Spectrum

The IVIS Spectrum is the newest imaging system available from Caliper Life Sciences. Like previous IVIS systems, it uses Xenogen’s novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

The IVIS Spectrum is the most versatile and advanced in vivo imaging system available on the market today. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas.

For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

For more information in our IVIS® Spectrum Series imaging system please click the button below and complete our product & services inquiry form.

or contact David Panzarella at +1-845-735-1921 or David.Panzarella@perkinelmer.com.

LabChip® XT

Advanced Nucleic Acid Size Selection and Collection
The LabChip XT fractionation system performs fast, automated nucleic acid fractionation accurately and reproducibly. The resulting sample is tightly sized and is delivered in a sequencing compatible buffer. The XT improves laboratory efficiency and provides sizing that is difficult to obtain using manual methods. Data is displayed digitally and non-fractionated sample can be recollected and used at another time. The XT has advanced extraction modes is ideal for users with more complex applications with data analysis and sample tracking needs.

Why Fractionate with the LabChip XT?
Current Next Generation Sequencing (NGS) workflows have numerous manual processes that bottleneck throughput and contribute to process inefficiency. One of the most time consuming tasks is gel-based size-selection during the library generation process. In addition to being labor intensive and not scalable, manual methods introduce run-to-run and operator-to-operator variability. The LabChip XT eliminates manual gel isolations from your sequencing library preparation.

• Reduce wasted “non-aligned” reads with tight size selectio
• Increase average read length by excluding shorter fragment
• Faster sample processing maximizes use of sequence
• Reduce waste and exposure to harmful reagent
• Fast, reproducible, high resolution DNA size selection

Advanced LabChip XT Software
The LabChip XT Instrument has all the functionalities of the XTe with added software features for sample tracking and data analysis. The quantitation feature allows for each sample’s total input concentration to be determined. Additionally, the estimated concentration of the extraction region is automatically calculated using Smear analysis. Sample Names can be imported and data can be automatically exported for LIMs compatibility.

Additional Extraction Mode
• Smear Max and Peak Max-Identify and extract in the region with maximum concentratio
• Skip Extraction- Identify and skip extraction of unwanted regions such as adapter dimer peaks
• Separation only-Utilize the analysis capabilities to perform nucleic acid quantitation without fractionation

Supporting Regulatory Compliance
The LabChip XT with LabChip GxP Software is a computerized system designed to automate the analysis and size selection of DNA. The systems allows the users to create, modify, and maintain the records in electronic form and allow the users to perform electronic signatures on the records generated from the system. It takes a combination of administrative controls, procedural controls, and technical controls for the users of a computerized system to comply with 21 CFR Part 11 regulations. The LabChip GxP Software contains built-in technical controls and features specifically designed to support the users for 21 CFR Part 11 compliance. These technical controls and features include user account management and access controls, device check, enforcing permitted sequencing of steps, audit trails, record copying, record retention, system documentation, and electronic signature controls.

Compliance Features
• User Account Managemen
• Audit trail
• Electronic Signature Control
• Central Data Repository

LabChip XT DNA300 Assay Kit (P/N 760601) and LabChip XT DNA750 Assay Kit (P/N 760541) sold separately.

MCF-7-luc-F5 Bioware® Cell Line

The MCF-7-luc-F5 Bioware® Cell Line (P/N 119262) is a light producing cell line derived from MCF-7 human mammary adenocarcinoma cells by stable transfection of the North American Firefly Luciferase gene expressed from the CMV promoter. Selection marker: Neomycin (G418 Sensitivity: 0.4 mg/ml).

Tissue: Human: adenocarcinoma, mammary gland

Parental Line Source: Karmanos Cancer Institute

Bioluminescence In Vitro: approx. 700 photons/sec/cell, subject to imaging and culturing conditions

In Vivo Models Tested:
Nude-beige (Harlan): mammary fat pad with lung metastasis
Nu/nu (CR): mammary fat pad

PC-3-luc2-GFP Bioware Ultra Green

PC-3-luc2-GFP is a luciferase and green fluorescent protein (GFP) expressing cell line. This cell line can be used in vivo to establish:

Orthotopic and subcutaneous tumor models
Ex vivo cell isolation using fluorescent activated cell sorter (FACS)
Post genomic analyses of isolated tumor cells.
Fluorescent detection of of GFP positive cells using Nuance Multispectral imaging systems

P/N: 133416

Designation: PC-3-luc2-GFP

Tissue: Human: Prostate; adenocarcinoma

Source of Parental Line: ATCC (CRL-1435™)

Bioluminescence In Vitro: Approximately 750 photons/sec/cell. Exact number will vary depending on imaging and culturing conditions.

Recommended Media: RPMI 1640 ATCC (Catalog Number 30-2001) Supplement with heat inactivated FBS at final concentration 10%

In vivo Models Tested: Nu/nu (CR); Subcutaneous

In vitro Luciferase and GFP stability

500,000 cells were plated per well in black-walled 24-well plates. Cells were incubated for 37C for recovery overnight and luciferase assay was performed using an IVIS Spectrum. Each experiment was done in quadruplicates. The assays were repeated over four weeks period of time. Bioluminescence data was analyzed using the Living Image 4.1 software.

Growth curve of 4T1-luc2-GFP

1 x10⁵ cells were plated on T25 flasks and the total numbers of cells were counted every 24 h using a Nexcelom automatic cell counter.

Subcutaneous Tumor Growth in a Nu/nu Mice

3 x 10⁶ PC-3-luc2-GFP cells were injected subcutaneously into the dorsal region near the thigh of female nu/nu mouse. Tumor growth was monitored by IVIS Spectrum at various time points for both luciferase and GFP expression. For luciferase expression, mice were imaged 10 minutes post i.p. injection of Luciferin at 150mg/kg at various time points. The image above shows tumor growth from representative mouse, while the chart shows tumor BLI signal from average of 8 animals. GFP signal from the tumor was measured using the ex 465/ em 520 nm filters.

ProfilerPro Glycan Profiling Kit

The new ProfilerPro Glycan Profiling Screening Assay designed for the LabChip GXII microfluidic system provides a fast, economically efficient means to assess the main glycoforms of IgG and related proteins. Caliper offers a complete set of 96 well plates and microfluidic assay components to enable the rapid digestion, dyeing, and identification of main glycans (N-Glycans) (charged glycans) in the Fc region of recombinant monoclonal antibodies (mAb).

The ProfilerPro Glycan Profiling Screening Assay is 60-70% faster, and 10-60% less expensive than competing technologies. Profiling of 96 samples can be performed in as little as half of a day.

Plates, Chips and Reagents sold separately:
• Glycan Release and Labeling Kit (P/N 760523)
• HT High Resolution LabChip (P/N 760524)
• HT Glycan LabChip Reagent Kit (P/N 760525)

LCGX v2.2 software or higher is required.

ProfilerPro Kinase Selectivity Assay Kit 1

ProfilerPro™ Kinase Selectivity Assay Kit 1 contains all necessary reagents and controls to carry out the rapid in-house screening and profiling of 24 protein kinases against up to 12 different compounds. Enzymes and fluorophore labeled peptide substrates are pre-dispensed into reaction-ready 384 well microtiter plates. Each kinase assay profiling kit will yield a predictable conversion of substrate (non-phosphopeptide) to product (phosphopeptide) at apparent ATP Km. Controls arrayed on the plate enable calculation of percent kinase inhibition of up to 12 customer selected compounds. ProfilerPro™ Kinase Selectivity Assay Kits are read using the Lab-on-a-Chip mobility shift assay technology and the EZ Reader, EZ Reader II or LabChip 3000.

Kit Components:

Component	Storage Temp
Enzyme Plate	-80°C
Substrate/ATP Plate	-80°C
Reconstitution Buffer	4°C
DTT	-80°C
Protease Inhibitor Cocktail	-80°C
Termination Buffer	4°C

Kinases in ProfilerPro Assay Kit 1

ABL	LCK
AKT1	LYNa
AKT2	MAPKAPK2
AurA	MAPKAPK5
CHK1	MET
CHK2	MSK1
CK1d	p38a
Erk1	PKACa
Erk2	PKC
FYN	PKD2
GSK3ß	RSK1
INSR	SRC

ProfilerPro Peptide 01

ProfilerPro Peptide 1 (P/N 760345) is a 5’-FAM labeled peptide substrate optimized for LabChip mobility shift kinase assays. ProfilerPro Peptide 1 has been validated in mobility shift kinase assays as a substrate IKKa, IKKb, PAK2, PKG1a, PKG1b and ROCK2 in LabChip kinase assays.

Label: 5'-FAM

Core Substrate Sequence: RRLSSLRA, additional amino acids available upon request.

Formulation: 1.5 mM

Storage: -20^oC

pXen05 Plasmid

pXen05 (pAUL-A Tn4001 luxABCDE Km^R)
This is a transposon-based lux delivery vector that contains a Gram-positive optimized luxABCDE cassette. The inverted repeats (IR) of the Tn4001 transposon are located at positions 305-332 and 8161-8188. The luciferase genes luxA (374-1453), luxB (1475-2455), luxC (2478-3917), luxD (3940-4860), luxE (4883-5992), and kanR (6016-6807) are co-transcribed.

General Purpose: Gram-positive lux transposon plasmid
Plasmid Size: 18359 bp
Antibiotic Selection: Erythromycin at 0.5 μg/ml
Growth Temperature: 37°C

This plasmid is a shuttle vector that will replicate in a range of Gram-positive and Gram-negative bacteria. However, amplification of this plasmid in E. coli will induce mutations and make the plasmid unstable. Thus, we recommend using the plasmid supplied from Caliper directly to transform Gram-positive bacteria.

Quantum FX uCT

Introducing Low Dose Imaging Designed for Longitudinal Studies

QUANTUM FX µCT

The Quantum FX system is the first stand alone μCT (micro CT) system to deliver high quality images at an X-ray dose low enough to enable true longitudinal μCT in preclinical studies. With Quantum FX you can follow and characterize disease progression throughout your complete study using μCT (microCT) at every imaging point. With an 18 second scan time that delivers a radiation doses less than most 2D X-ray imagers, you can be confident that your biological model will remain relevant throughout the span of your experiment. Fast imaging and smooth workflow also enable the throughput required to scan cohorts of animals quickly and draw sound conclusions from your experimental data.

or contact David Panzarella at +1-845-735-1921 or David.Panzarella@perkinelmer.com

Sciclone NGS Workstation

The Sciclone NGS (Next Generation Sequencing) Workstation (P/N SG3-31020-0300) is the most capable automated solution for high-throughput sequencing sample preparation. Built on the Sciclone G3 platform, the NGS Workstation is a complete benchtop solution for library prep, sequence capture, and normalization.

• 480 libraries per week
• 192 exome captures per week
• Flexibility to run 8-96 samples per run
• Intuitive user interface
• Support for multiple protocols and kits
• Sequence capture, mRNA-Seq, ChIP-Seq
• Sample indexing and normalization

Staccato iBLOX Modular System Tables

The Staccato Series of systems provide fast and reliable scalable automation for today's drug discovery, genomics, proteomics and drug development laboratories. Staccato systems are available in three base configurations; Mini Workstation Series, Application Series and Custom Systems Series.

All Staccato Application Series systems use a revolutionary new base table design called iBLOX™. iBLOX™ are modular tables that can be interconnected to form a variety of system configurations, using just three basic foundations, (A-Block, B-Block and C-Block). Each table comes with casters, allowing them to be easily rolled about, set in place and quickly leveled for operation. iBLOX™ provide "usable scalability" in that one can start with just a Sciclone liquid handler on an "A Block" and add additional automation at a later time, with no reconfiguration or loss of initial investment.

All Staccato iBLOX based systems are pre-integrated and arrive mostly assembled, requiring minimal installation. All system components are pre-mounted and all device locations are taught at the factory. Automated teaching routines are included allowing the system to be re-taught in the field in less than five minutes. Methods are easily and quickly created using Caliper's industry leading iLink software. Caliper's Applied Science & Technology Group works with a variety of reagent and consumables manufactures to pre-validate common assays for all Caliper systems.

TRIO™

Simplified multi-label imaging of single tissue sections

TRIO™ provides quick, simple and affordable microscopy imaging in both brightfield and fluorescence. Up to three chromogens or up to three fluorophores can be captured simultaneously in a single tissue sample - delivering superior performance over filter-based systems. TRIO also offers multi-labeled per-cell measures of nuclear, cytoplasmic, and membranous protein expression, making it ideal for monitoring protein signaling pathways and resolving protein co-expression.

The system is as straightforward to use as a point-and-shoot camera - advanced knowledge of multispectral imaging is not needed. TRIO is compatible with any microscope that has a camera mount and is easily setup in just a few minutes, enabling users to start capturing images almost immediately. TRIO provides capture of multi-labeled images, high-resolution color images for annotation and publishing, and automatically exports standard image file formats for further image analysis.

TRIO fluorescence images of liver. Note the absence of crosstalk and the high image quality.

inForm advanced image analysis software is also an available option for TRIO. The ability to train the software to analyze only user-selected regions provides a powerful solution for performing nuclear, cytoplasmic, and membrane measurements on individual cells.

TRIO Specifications

Modality Fluorescence and Brightfield

Spectral Range (Brightfield) 440-700 nm

Spectral Range (Fluorescence) 440-480 nm (Blue channel)
500-560 nm (Green channel)
600-660 nm (Red channel)

Brightfield Labels Any 3 chromogens

Fluorescent Labels Blue: DAPI, Hoechst
Green: FITC, AF488, eGFP, QDot 525
Red: Texas Red, AF594, QDot625

Analysis Capability Optional

iNForm Capability Yes

XenoLight D-Luciferin Potassium Salt

Luciferin is a chemical substance found in the cells of various bioluminescent organisms. When luciferin is oxidized under the catalytic effects of luciferase and ATP, a bluish-green light is produced. Because the reaction is dependent on ATP, it allows researchers to determine the presence of energy or life. Firefly luciferin is a particularly good reporter for in vivo biophotonic imaging due to properties of its emission spectra.

D-Luciferin Potassium Salt (P/N 122796) is isolated from firefly and extensively validated in a number of biophotonic imaging applications using the IVIS^® Imaging System available from Caliper Life Sciences.

Luciferin Toxicity

Luciferin is a low molecular weight organic compound that consists of a benzothiazole moiety attached to a thiazole carboxylic acid moiety. Luciferin is found in fireflies and other animals which, in the presence of ATP and the enzyme luciferase, becomes luminescent. The small size of luciferin also makes it a poor antigen and immune responses to luciferin are unlikely. Luciferin is able to pass the blood brain barrier, the blood placenta barrier and the blood testis barrier, toxicity appears low.

Frequently asked questions

How do you administer luciferin?
Mice with lux-bearing bacteria do not need luciferin to glow. In the tumor models and transgenic models, luciferin is administered intraperitoneally (concomitant with anesthesia).

How well does luciferin distribute?
Luciferin distributes quickly and easily throughout the animal.

How do the animals respond to the repeated administration of luciferin substrate?
Luciferin does not affect the animals deleteriously (no evidence of toxicological or immunological effects).

Do you need to administer luciferin substrate to the animals before imaging?
In bacteria, the entire luciferase operon is stably integrated on the chromosome. This eliminates the need for exogenous luciferin substrate in the bacterial models. The tumor models and transgenic models rely on the exogenous administration of luciferin.

XenoLight RediJect D-Luciferin

Part Number: 760504

RediJect D-Luciferin (K+ salt) is a bioluminescence substrate in a ready to use pre-formulated injectable format developed by the in vivo imaging leaders to fit your laboratory workflow. With Caliper’s Rediject D-Luciferin you can ensure a reproducible result by minimizing variability in formulation, minimizing freeze/thaw and controlling batch to batch variation. With over 1,000 publications and millions of mice imaged, Caliper has formulated its substrates to fit common workflow patterns and optimize results. You are free to concentrate on animal handling and biology.

Zephyr® Molecular Biology Workstation

A robust and simple solution for DNA and RNA applications.

Ideal for:

• DNA extraction	• Sequencing purification
• RNA extraction	• PCR setup
• Plasmid isolation	• Sequencing reaction setup
• PCR purification	• Normalization
• PCR sequencing	• More…

The Zephyr Molecular Biology Workstation is a powerful solution for automating numerous critical yet routine molecular biology processes such as nucleic acid purification, reaction setup, and normalization. This benchtop workstation is designed to remove many of the technical hurdles of automating these protocols, without sacrificing functionality and flexibility.

Advances in genomics have generated a clear need for higher sample volumes without sacrificing data quality. Automating many of the error prone and routine manual protocols is the logical path, but unfortunately automation often comes with its own challenges, such as learning to program methods for machine execution and planning assays for unattended operation.

The Zephyr Molecular Biology Workstation is the perfect compliment for today’s molecular biology laboratories. Whether isolating total RNA, purifying PCR fragments, setting up reactions, or normalizing DNA samples, the Zephyr Molecular Biology Workstation provides a straight forward and simplified approach, which reduces errors and ensures reproducibility.

Click here to access our Zephyr MB Methods

Zephyr® SPE Workstation

The Zephyr SPE Workstation is a simple and efficient, yet powerful tool for automating the condition, load rinse and elute steps required in Solid Phase Extraction (SPE). The workstation is designed to specifically meet the needs of clinical, forensic, and pharmaceutical/biotech laboratories doing high-throughput SPE in a 96 well plate. The Zephyr SPE system is an automated solution for sample preparation, cleanup, and extraction with the ability to process 96 samples simultaneously in 20-40 minutes, depending on the method.

Additionally, the Zephyr SPE is equipped with a truly unique 'Clog Detection' * feature called “PING!”. By using a state of the art ultrasonic sensor, the Zephyr SPE Workstation can quickly examine all wells on a plate to determine if the wells have emptied before moving on to the next step of the SPE method. If a clog well is detected, the workstation can apply vacuum for additional time or notify the operator by e-mail (if the computer is on the network) and put up an error screen showing what well(s) are clogged. Once the clog is resolved, the workstation can complete the SPE method. This feature provides the operator true walk away time, and the knowledge that there is no risk of contaminating the SPE plate if a well is clogged and additional reagents are added to that well. The “PING!” Clog Detection feature makes the Zephyr SPE Workstation a powerful sample preparation tool.

The Zephyr SPE workstation in addition to providing the “PING!” sensor to assure no wells are clogged, and allowing for “unattended” walk away time has a software interface tailored specifically to SPE and other sample clean-up techniques. There is no need to be a “programmer” or “expert liquid handler”, because our software lets you set-up a method with only 3 simple screens, and uses steps of condition, load, rinse and elute, and not load tips, aspirate, dispense.

* Patent Pending

TRIO Specifications
Modality	Fluorescence and Brightfield
Spectral Range (Brightfield)	440-700 nm
Spectral Range (Fluorescence)	440-480 nm (Blue channel) 500-560 nm (Green channel) 600-660 nm (Red channel)
Brightfield Labels	Any 3 chromogens
Fluorescent Labels	Blue: DAPI, Hoechst Green: FITC, AF488, eGFP, QDot 525 Red: Texas Red, AF594, QDot625
Analysis Capability	Optional
iNForm Capability	Yes

Featured Products

iSTAX® Plate Stacking Solutions

Plate Stackers

IVIS® Kinetic

IVIS® Spectrum

LabChip® XT

MCF-7-luc-F5 Bioware® Cell Line

PC-3-luc2-GFP Bioware Ultra Green

In vitro Luciferase and GFP stability

Growth curve of 4T1-luc2-GFP

ProfilerPro Glycan Profiling Kit

ProfilerPro Kinase Selectivity Assay Kit 1

Kit Components:

ProfilerPro Peptide 01

pXen05 Plasmid

Quantum FX uCT

Introducing Low Dose Imaging Designed for Longitudinal Studies

Sciclone NGS Workstation

Staccato iBLOX Modular System Tables

TRIO™

Simplified multi-label imaging of single tissue sections

XenoLight D-Luciferin Potassium Salt

Frequently asked questions

XenoLight RediJect D-Luciferin

Zephyr® Molecular Biology Workstation

Zephyr® SPE Workstation

Zephyr® SPE Workstation