Software
Version: LabChip GX V2.2 SP2
P/N 127773 Rev 00
The LabChip GX software controls the LabChip GX and LabChip GX II instruments. The LabChip GX software is also used to analyze and compare data in the form of gels or graphs across different wells from the same or different LabChip GX data files. The software can also compare data with similar characteristics by applying filters. Results can be exported to graphic files or .csv (comma separated values) files. The software can be installed in Reviewer mode to be used only for data analysis. The 21 CFR Part 11 option can be installed on the LabChip GX computer to comply with 21 CFR Part 11 requirements. The 21 CFR Part 11 option supports remote data backup to a database on a network server. Use the CDR Server Utility software to set up the remote server.
U.S. English Version of Microsoft© Windows XP Service Pack 2 or higher
Minimum RAM: 1Gb (2Gb recommended)
U.S. English Version of Microsoft® Windows XP Service Pack 2 or higher, OR Microsoft® Windows Vista
Minimum RAM: 1Gb (2Gb recommended)
U.S. English Version of Microsoft® Windows XP Service Pack 2 or higher, OR Microsoft® Windows Vista
Available Hard Disk Space: 10 - 20Gb (depending on use)
Minimum RAM: 1Gb (2Gb recommended)
To install the LabChip GX software on Windows XP:
1. Close all open programs.
2. Insert the LabChip GX Installation CD into the CD-ROM drive.
3. Follow the instructions in the setup wizard.
If the installation does not automatically start:
1. From the Start menu, select Run.
2. Type d:\setup.exe (or type the drive letter of the CD-ROM drive).
3. Click the OK button.
4. Follow the instructions in the InstallShield wizard.
To install the LabChip GX software on Windows Vista (Reviewer Mode ONLY):
1. Close all open programs.
2. In Windows Explorer, right-click on setup.exe and select “Run as administrator.”
3. In the Warning dialog, select the Allow option. The InstallShield Wizard displays the list of prerequisites.
4. Click the Install button to install the required prerequisites. The LabChip GX InstallShield Wizard opens.
5. Follow the instructions in the InstallShield wizard.
To install or set up the remote CDR, you must log onto the Remote Server computer as a Windows administrator.
For security purposes, files and folders on the remote server pc should not be shared or accessible by regular users on the network.
If Subversion V1.5 or higher is installed on the remote server, a dialog prompts you to manually uninstall subversion. Installation will not continue until Subversion is uninstalled. After manually uninstalling Subversion, restart the installation.
To install the CDR Server Utility software on Windows XP:
1. In Windows Explorer, double-click on CDRServerUtilitySetup.exe.
2. Follow the instructions in the InstallShield wizard.
3. If this is the first time the CDR Server Utility is installed, you must restart the computer.
To install the CDR Server Utility software on Windows Vista:
1. In Windows Explorer, right-click on CDRServerUtilitySetup.exe and select “Run as administrator.”
2. In the Warning dialog, select the Allow option. The CDR Server Utility InstallShield Wizard opens.
3. Follow the instructions in the InstallShield wizard.
4. If this is the first time the CDR Server Utility is installed, you must restart the computer.
To start the CDR Server Utility software, right-click on the desktop icon and select ‘Run as Administrator.’
Troubleshooting Remote CDR Server installation:
1. After creating the server, verify the windows service ‘Subversion’ is running.
2. The Remote and Local computers should be in the same workgroup. If they are not in same workgroup, use the IP Address instead of the computer name when specifying the remote CDR server on the LabChip GX computer.
3. After creating the server and folder, if the Get Folder Details check box was selected, the server name, folder name, username, and password are saved in a text file in “C:\Program Files\Caliper Life Sciences\CDRServer\ServerDetails”. This information is required for backing up the local folder on the LabChip GX computer to the remote CDR folder.
To uninstall the LabChip GX software:
1. From the Start menu, select Control Panel.
2. Double click on Add or Remove Programs in the Control Panel.
3. Select LabChip GX from the list of installed programs.
4. Click on the Remove button to uninstall the LabChip GX software.
Resolved Issues:
· The ability to Scale to Sample Peaks was not available after modifying smear baselines (LCF-4306). Adjusting smear baselines will no longer affect this feature.
· Overlapping smears were forced to be non-overlapping when manual overrides were reset (LCF-4304). This constraint is no longer applied to smears.
· When a smear baseline was overridden, the concentration for the smear would be reported as 0 (LCF-4309). This problem has been fixed.
· The focus algorithm has been improved to reduce focus failures due to noise. (LCF-4034)
· If a manual peak is added in a region which, after analysis settings changes, becomes identified as a peak, two peaks would appear overlapping in the same area and that area would be counted twice. The manual peak will now disappear if its center is contained within a peak found by analysis. (LCF-4258)
· Smears were being normalized with raw marker area instead of aligned area resulting in small inaccuracy in smear concentration calculation (LCF-4286). Aligned areas will now be used for normalization. This change also affects Protein Corrected Area which will now be derived from aligned area as is appropriate and thus will change slightly in peak table. This change will not affect peak concentrations or %Purity as these quantities are based on area ratios.
· Manual integration limit changes would seem to get lost in a specific scenario where the boundary between two abutting peaks was being moved to the right. The right limit of the left peak was being pushed back to the original limit of the right peak upon reanalysis. The left peak right limit will now be constrained to abut with the adjusted left limit of the right peak rather than the original limit.
· The property “Size at Maximum” is now being computed for smears (LCF-4297). The value of this property is the size where the smear attains its maximum height from the baseline. View this property by selecting it in the Property Displayed in Well Table column of the Smear Analysis table in Analysis Settings.
Assay Changes:
· HT Glycan.asy – The priming script was changed to perform the prime in four short steps instead of two long steps to avoid running out of range on the pump observed on some instruments. (LCF-4055)
Resolved Issues:
· In 21 CFR Part 11 Compliance mode, the LabChip GX now only opens data files in the CDR (Central Data Repository) – Opening a workspace that contains data files that are not saved in the CDR generates an error message. Use a non-CFR version of the LabChip GX software to open non-CFR workspaces and data files. (LCF-3768)
· Resolved error in Chip ID when writing to RFTag – When writing to the RFTag, the first character of the Chip ID may be written as the first character of the previous chip if the RFTag was not read before writing. This issue has been resolved. (LCF-3818)
· Signals in GX V2.2 are one second faster than in previous versions of the LabChip GX software – In V2.2 firmware a one second delay in the initialization was introduced which leads to the discrepancy of the data timestamp. (LCF-3877)
Assay Changes:
· HT DNA 1K.asy - Changed Peak Sizing from Leading Edge to Center.
· HT Glycan.asy - Increased time parameters (Start Time, End Time, Baseline Start, Well Total) by 1 second to match firmware fix to time offset.
New Features:
· Glycan Assay – New assay for identifying and measuring relative quantities of Glycans on LabChip GX II instruments. The Glycan ladder is a series of molecules assembled as multiples of a primitive Glycan molecule = 1 Caliper Glycan Unit [CGU].
· Default Ladder – Provides ability to select a ladder as the default ladder that can be applied to any group of wells in the plate. Default Ladder is exported in assays and can be used in subsequent runs.
· Column-wise Sipping – Provides ability to select the order in which the wells are sampled. Select Column-wise in the Start Run window to sample by column (A1, B1, C1…). Select Row-wise in the Start Run window to sample by row (A1, A2, A3…). (Ladders are sipped after every 12 wells, regardless of the sipping order.)
· Titer Analysis Improvements – Ability to select either Linear fit or Quadratic Fit for Titer Curve. Force Fit Through Origin option now available to force Titer Curve through (0,0) to improve accuracy of calculations for small peaks.
· Filter Legend in Gel View – Show Filter Legend in Gel Properties provides ability to display a legend of Filter colors in Gel View.
· Include Peak Option – Ability to include peaks outside marker (Peak Type is “?”), which are normally excluded. Included peak will be quantified and included in sample peak area/concentration totals.
· Improved Column Selection in Peak Table and Well Table – Right-clicking on the column header in the Peak Table View or Well Table View opens the Select Peak/Well Table Columns window opens to choose the columns to display in the table.
· Raw Data Export in NetCDF Format – Enables export of Raw Data in NetCDF Format (formerly AIA format). Generates one .cdf file for each selected well.
· Print Improvements – Add Border option now available for Peak Table or Well Table. Adds borders between columns and wells in printed tables. Well Header option available for Peak Table to add well identifier above peak table for each well. Column Header option available for Peak Tables. Per Page option repeats column headings at the top of each page. Per Well option repeats column headings at the beginning of each well. When Peak and Well Tables are printed together, the tables are combined and printed on one page per well.
Known Issues:
Open Issues in LabChip GX V2.2:
· Plates sipped with LabChip GX V2.2 software using column-wise sipping order do not display some ladders when viewed in LabChip GX V2.1 software. (LCF-3577)
· Default Ladder is identified as “Ladder 9” in the Version Log. (LCF-3553)
· All viewers are hidden if the LabChip GX V2.2 software is minimized during the run. Do not minimize the software during a run. (LCF-3546)
· Front panel buttons may not function after terminating the software using CalTaskClean. Restart the instrument or restart the LabChip GX software to reactivate the front panel buttons on the instrument. (LCF-1828)
· CalTaskClean closes any open document or window that starts with "LabChipGX", not just the LabChip GX software. (LCF-3418)
· Selected peak in peak table may not be visible in overlay electropherogram if there are other peaks near it. (LCF-3273)
· "USB Key not found" error may occur while starting the software if the USB cable is disconnected in between a run and then connected. Workaround: Unplug the cable again, plug it back in, wait a few seconds, and then restart the software. (LCF-3401)
Complete documentation is available in the LabChip GX.chm HTML Help file (accessible by selecting Helpŕ LabChip GX Help in the software) or in the LabChip GX User Manual.pdf.
Copyright (c) 2008 - 2010, Caliper Life Sciences. All rights reserved.
Contact Caliper Technical Support by phone, fax, or e- mail from 8AM to 5PM Eastern Standard Time.
Telephone: 1-877-LABCHIP (1-877-522-2447), toll free
Fax: (508) 435-3439
E-mail: tech.support@caliperLS.com
Internet Address: http://www.caliperLS.com