4T1-luc2-GFP Bioware® Ultra Green
4T1-luc2-GFP is a luciferase and green fluorescent protein (GFP) expressing cell line. This cell line can be used in vivo to establish:
- Mammary fat pad orthotopic tumor models
- Subcutaneous tumor models
- Ex vivo cell isolation using fluorescent activated cell sorter (FACS)
- Post genomic analyses of isolated tumor cells.
- Immunohistochemistry detection of GFP positive cells
P/N: 128090
Designation: 4T1-luc2-GFP
Tissue: Mouse: mammary gland; adenocarcinoma
Source of Parental Line: ATCC (CRL-2539™)
Bioluminescence In Vitro: Approximately 170 photons/sec/cell. Exact number will vary depending on imaging and culturing conditions.
Recommended Media: RPMI 1640 ATCC (Catalog Number 30-2001) Supplement with heat inactivated FBS at final concentration 10%
In vivo Models Tested: Nu/nu (CR); Subcutaneous
In vitro Luciferase and GFP stability
500,000 cells were plated per well in black-walled 24-well plates. Cells were incubated for 37C for recovery overnight and luciferase assay was performed using an IVIS Spectrum. Each experiment was done in quadruplicates. The assays were repeated over four weeks period of time. Bioluminescence data was analyzed using the Living Image 4.0 software.
Growth curve of 4T1-luc2-GFP
1 x105 cells were plated on T25 flasks and the total numbers of cells were counted every 24 h using a Nexcelom automatic cell counter.
Subcutaneous Tumor Growth in a Nu/nu Mice
3 x 106 4T1-luc2-GFP cells were injected subcutaneously into the dorsal region near the thigh of female nu/nu mouse. Tumor growth was monitored by IVIS Spectrum at various time points for both luciferase and GFP expression. For luciferase expression, mice were imaged 10 minutes post i.p. injection of Luciferin at 150mg/kg at various time points. The image above shows tumor growth from representative mouse, while the chart shows tumor BLI signal from average of 3 animals. GFP signal from the tumor was measured using the ex 465/ em 520 nm filters.