Epigenetic Assays
Quick Links: | HTS | IC50 Evaluations | MoA Studies | Assay List
CDAS EpiScreen is a comprehensive suite of epigenetic targets services designed to support hit discovery and lead optimization efforts. Most of our assays are developed in a Caliper assay LabChip® format and are fully optimized to deliver superior data quality and excellent data reproducibility.
HTS
• Screening conditions tailored to your needs (plate layouts, control wells, etc.)
• High QA & QC standards (postive and negative controls run throughout all the plates)
• Automated compound cherry picking capabilities for hit compound confirmation
• Complete standard or customized report for seamless integration into in-house databases (IDBS…)
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IC50 Evaluation - 12 point or custom duplicate IC50 curves
• Choice of dose range of concentrations
• Quality control: reference compound IC50 determination and comparison to historical database
• Low amount of compound required
• Turnaround time: 2 weeks
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Mechanism of Action (MoA) Studies
Provides detailed information about the manner in which a drug interacts with enzymatic targets. MoA studies and particularly important for characterizing the specific type of enzyme inhibition and for assessing kinetic on and off rates.
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LabChip Technology platform
CDAS conducts most of its EpiScreen services using the LabChip microfluidic technology developed by Caliper Life Sciences.
Key features and benefits of the technology:
• No monoclonal antibodies or radioisotopes are required
• Detection of biochemical inhibition in a direct, homogenous, ratiometric, high quality format
- substrate and product are measured in every well
The separation and direct measurement of substrates and products allowing:
• Higher signal-to-noise ratios
• Fewer false positive and negative results
• Identification & elimination of enzymatic activities not associated with the kinase reaction of interest
• The ability to read over time (kinetic format)
A small reaction volume is used for the detection of substrate and product:
• Minimum sample requirements (100 ug) is enough for profiling a compound in a panel of assays
• Profile your new lead compounds and other chemicals faster at a lower cost
• Due to minimal sample requirements you save your compound for additional CDAS in vitro studies
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EpiScreen Assay List
|
Cat# |
Assay Type |
Assay Class |
Assay Name |
$/Well (USD) |
|
Enzyme |
Deacetylase |
Histone, HDAC3 (h) |
$95.00 |
|
|
Enzyme |
Deacetylase |
Histone, HDAC6 (h) |
$95.00 |
|
|
Enzyme |
Deacetylase |
Histone, HDAC8 (h) |
$95.00 |
|
|
Enzyme |
Deacetylase |
Sirtuin, Sirt1 (h) |
$95.00 |
|
|
Enzyme |
Deacetylase |
Sirtuin, Sirt2 (h) |
$95.00 |
|
|
Enzyme |
Deacetylase |
Sirtuin, Sirt3 (h) |
$95.00 |
|
|
Enzyme |
Deubiquitinase |
BAP1 (h) |
$90.00 |
|
|
Enzyme |
Deubiquitinase |
UCH-L1 (h) |
$90.00 |
|
|
Enzyme |
Deubiquitinase |
UCH-L3 (h) |
$90.00 |
|
|
Enzyme |
Deubiquitinase |
USP5 (h) |
$90.00 |
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