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c-Kit (h)

Cat#: 200-0418
Species: Human
RegionRecombinant cells
Enzyme FamilyTyrosine kinase (TK)
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)7.32E-04
Km (Peptide Substrate)1.1E-05
ATP [M]7.32E-04
ReactionFluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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