Kinase, Protein, cRAF (RAF1) (h)
| Cat#: | 200-0774 |
| Species: | Human |
| Region | Recombinant cells |
| Enzyme Family | Tyrosine kinase like (TKL) |
| Substrate | Fluorescein-labeled peptide |
| Substrate [M] | 1.5E-6 |
| Km (ATP) | 6E-6 |
| Km (Peptide Substrate) | 3.5E-05 |
| ATP [M] | 6E-6 |
| Reaction | Fluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP |
| Method | Fluorescence – electrophoretic mobility shift |
| Measurement | Separation of phosphorylated and unphosphorylated substrate |
| Reference Compound | SB203580
IC50 = 421 nM |
