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Kinase, Protein, Flt3 (D835Y) (h)

Cat#: 200-0831
Species: Human
RegionRecombinant cells
Enzyme FamilyTyrosine kinase (TK)
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)9.00E-06
Km (Peptide Substrate)1.6E-05
ATP [M]9.00E-06
ReactionFluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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