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Kinase, Protein, GSK-3alpha (h)

Cat#: 200-0424
Species: Human
RegionRecombinant cells
Enzyme FamilyCyclin dependent kinase containing CDK, MAPK, GSK3, & CLK families (CMGC)
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)2.90E-05
Km (Peptide Substrate)6.8E-05
ATP [M]2.90E-05
ReactionFluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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