Kinase, Protein, MAPK1 (ERK2, p42mapk) (h)
| Cat#: | 200-0747 |
| Species: | Human |
| Region | Recombinant cells |
| Enzyme Family | Cyclin dependent kinase containing CDK, MAPK, GSK3, & CLK families (CMGC) |
| Substrate | Fluorescein-labeled peptide |
| Substrate [M] | 1.5E-6 |
| Km (ATP) | 1.80E-05 |
| Km (Peptide Substrate) | 2.4E-04 |
| ATP [M] | 1.80E-05 |
| Reaction | Fluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP |
| Method | Fluorescence – electrophoretic mobility shift |
| Measurement | Separation of phosphorylated and unphosphorylated substrate |
| Reference Compound | K252a IC50 = 36 nM |
