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Kinase, Protein, PAK4 (h)

Cat#: 200-0916
Species: Human
RegionHuman recombinant, Catalytic Domain (295-591)
Enzyme FamilySerine/Threonine Kinase
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-06M
Km (ATP)8.4E-06M
Km (Peptide Substrate)1.4E-06M
ATP [M]8.4E-06M
ReactionFluorescein -peptide + ATP -> fluorescein -phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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