Kinase, Protein, ROCK1 (h)
| Cat#: | 200-0919 |
| Species: | Human |
| Region | Human recombinant, Catalytic Domain (1-477) |
| Enzyme Family | Serine/Threonine Kinase |
| Substrate | Fluorescein-labeled peptide |
| Substrate [M] | 1.5E-6M |
| Km (ATP) | 6E-6M |
| Km (Peptide Substrate) | 17E-6M |
| ATP [M] | 1.5E-6M |
| Reaction | Fluorescein -peptide + ATP -> fluorescein -phosphopeptide + ADP |
| Method | Fluorescence – electrophoretic mobility shift |
| Measurement | Separation of phosphorylated and unphosphorylated substrate |
| Reference Compound | Staurosporine |
