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Kinase, Protein, YES (h)

Cat#: 200-0935
Species: Human
RegionHuman recombinant, full-length
Enzyme FamilyTyrosine Kinase
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)1.35E-4
Km (Peptide Substrate)2.3E-5
ATP [M]1.35E-4
ReactionFluorescein-Peptide + ATP -> fluorescein-Phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated product and unphosphorylated substrate
Reference CompoundStaurosporine
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