Deacetylase, Sirtuin, Sirt1 (h)
| Cat#: | 200-0926 |
| Species: | Human |
| Region | Human recombinant |
| Enzyme Family | Class 3 HDAC |
| Substrate | Fluorescein-labeled peptide |
| Substrate [M] | 3.0E-6 |
| Km (enzyme affinity) | Km (NAD) = 640E-6 M Km (Peptide) = 18E-6 M |
| Reaction | Fluorescein - Acetylated peptide substrate -> Deacetylated peptide product |
| Method | Fluorescence – electrophoretic mobility shift |
| Measurement | Ratiometry of acetylated substrate and deacetylated product |
| Reference Compound | Suramin |
