Discovery 1 X-MAN
Single Mutated Cancer Gene Panel
• Primary druggable mutant vs. normal human cancer genotypes
• 25+ single KI/KO cell-lines
• Target selectivity; target ID, biomarkers, patient-relevance
• X-MAN Express protein & mRNA lysates
| Cancer Gene | Parental Cell | Notes |
| PI3Ka (H1047R) | Normal Breast Epithelium | Activating kinase domain mutation. |
| PI3Ka (E545K) | Normal Breast Epithelium | Activating hinge domain mutation. |
| EGFR (del E746-A750) | Normal Breast Epithelium | Activating kinase domain mutation. |
| P13Ka (E545K) | SW48 Colon Cancer Cells | Activating hinge domain mutation. |
| K-Ras (G13D) | SW48 Colon Cancer Cells | GTPase mutant leading to constitutive Ras-signalling |
| B-Raf (V600E) | SW48 Colon Cancer Cells | Activating kinase domain mutation. |
| p53 null | SW48 Colon Cancer Cells | Tumour suppressor KO |
| p53 null | HCT-116 Colon Cancer Cells | Tumour suppressor KO |
| p53 (R248W/+) mutant KI | HCT-116 Colon Cancer Cells | KI of mutant tumour suppressor |
| p53 (R248W/-) | HCT-116 Colon Cancer Cells | KI/KO ofmutant tumour supressor |
| PTEN null | HCT-116 Colon Cancer Cells | Tumour suppressor KO |
| SMAD4 null | HCT-116 Colon Cancer Cells | Tumour suppressor KO |
| MLH1 (WT knock-in) | HCT-116 Colon Cancer Cells | KI of wild-type gene in mutant parent cells |
| P13Ka (H1047R KO) | HCT-116 Colon Cancer Cells | KO of mutant allele |
| K-Ras (G13D KO) | HCT-116 Colon Cancer Cells | KO of mutant allele (GFP version available) |
| B-Catenin (^45 KO) | HCT-116 Colon Cancer Cells | KO of mutant allele |
| P13Ka (E545K KO) | DLD1 Colon Cancer Cells | KO of mutant allele |
| K-Ras (G13D KO) | DLD1 Colon Cancer Cells | KO of mutant allele (GFP version available) |
| p53 (S241/silenced) | DLD1 Colon Cancer Cells | Parental DLD-1 is mutant/silenced |
| p53 (-/silenced) | DLD1 Colon Cancer Cells | KO of mutant allele (S241) (2nd Allele still silenced) |
| p53 (WT/silenced) | DLD1 Colon Cancer Cells | KI of WT p53 into mutant allele (2nd Allele still silenced). |
| p53 (S241/-) | DLD1 Colon Cancer Cells | KO of silenced p53 allele |
| BRCA2 null | DLD1 Colon Cancer Cells | KO of DNA repair 'caretaker' gene |
| BRCA2 KI | DLD1 Colon Cancer Cells | KI of various BRCA2 point mutations in (+/-) parent cells |
| p53 (R248W/+)mutant KI | RKO Colon Cancer Cells | KI of mutant tumour supressor |
| p53 null | RKO Colon Cancer Cells | Tumour suppressor KO |
| B-Raf (V600E) mutant KO | RKO Colon Cancer Cells | KO of mutant allele |
| B-Raf (V600E) mutant KO | VACO Colon Cancer Cells | KO of mutant allele |
| MMSET | Human Myeloma Cell line. | KO of translocated MMSET allele in a Multiple Myeloma line |
