HT Protein Express LabChip® 90 Assay Kit
Overview
The HT Protein Expression 200 LabChip assay kit designed for use on the LabChip 90 provides a fast and easy way to analyze a wide range of protein samples ranging from 14kDa to 200kDa. Running protein samples with the Protein Express assay saves time and money by automating steps for protein analysis, and replaces SDS-PAGE slab gels, staining, and imaging.The LabChip 90 Electrophoresis System uses a single sipper microfluidic chip to aspirate denatured protein samples directly from 96- or 384-well plates. The microfluidics chip technology automatically stains, electrophoretically separates, and destains the protein sample. The instrument optics detect the laser-induced fluorescent protein signal. System software automatically analyzes the data and determines protein size and concentration relative to a ladder and marker calibration standard. Digital data results are immediately available for review or reporting in virtual gel, electropherogram graph, or table summary form.
Applications
• Protein Expression/Analysis (Presentation)
Features
• Save Time! – High throughput provides complete automated analysis of protein samples in under 40 seconds
• Save Money! – Automated staining, destaining, and analysis procedure provides significant cost savings relative to the materials and labor required to run manual gels for protein gel electrophoresis
• Increase Experimental Opportunites! – Eliminate the resource bottleneck which can constrain protein expression & optimization studies
• Analyze a large variety of protein samples - Cell lysates, column fractions, antibodies and purified proteins
• Automated exporting of results
• Digital format facilitates review, export, and archiving of data
• Extended Workflow profile allows chip to be loaded once for support of sample processing anytime within an 8-hour window
• Flexible data display options - Results shown in your choice of virtual gel, electropherogram graph, or tabular formats
• Save Money! – Automated staining, destaining, and analysis procedure provides significant cost savings relative to the materials and labor required to run manual gels for protein gel electrophoresis
• Increase Experimental Opportunites! – Eliminate the resource bottleneck which can constrain protein expression & optimization studies
• Analyze a large variety of protein samples - Cell lysates, column fractions, antibodies and purified proteins
• Automated exporting of results
• Digital format facilitates review, export, and archiving of data
• Extended Workflow profile allows chip to be loaded once for support of sample processing anytime within an 8-hour window
• Flexible data display options - Results shown in your choice of virtual gel, electropherogram graph, or tabular formats
Demos
Our product can be demonstrated at our facility in Hopkinton, Massachusetts or other Caliper Life Sciences offices worldwide. Please contact your local Caliper Representative to arrange a demo.
Specifications
Size Range |
|
| 14kDa – 200kDa | |
Linear Range |
|
| 5.0 – 2000 ng/ul | |
Analysis Time per Sample |
|
| 39 seconds | |
Sizing Accuracy |
|
| +/- 20% | |
Resolution |
|
| 10% difference in MW across the sizing range, approx comparable to a 10cm 4-20% SDS-PAGE gel | |
Sensitivity |
|
| 5.0 ng/ul Carbonic Anhydrase, approx comparable to a mid-range colloidal Coomassie stain |
