HT RNA LabChip® 90 Assay Kit
Overview
The HT RNA assay kits designed for use on the LabChip 90 provides a fast and easy way to analyze RNA samples ranging in size from 100 to 6,000 nucleotides. Performing RNA sample analysis with the HT RNA assay kit saves time and money by automating steps for quantitation and integrity analysis. HT RNA provides the user with RNA concentration and ribosomal ratios as an indicator of integrity.The LabChip 90 Electrophoresis System uses a single sipper microfluidic chip to aspirate RNA samples directly from 96- or 384-well plates. The microfluidics chip technology automatically mixes in an intercolating dye*, electrophoretically separates, and analyzes the RNA sample. The instrument optics detect the laser-induced fluorescent signal. System software automatically analyzes the data and determines fragment size and concentration using ladder and marker calibration standards. Digital data results are immediately available for review or reporting in virtual gel, electropherogram graph, or table summary form.
* Licensed under patents and patent applications owned by Molecular Probes, Inc.
Applications
RNA Gene Expression QC (Presentation)
Features
• Save Time! – High throughput provides complete automated analysis of RNA in about 80 seconds
• Save Money! – Automated analysis procedure provides significant cost savings relative to the materials and labor required to run manual gels for RNA analysis
• Automated exporting of results
• Digital format facilitates review, export, and archiving of data
• Extended Workflow profile allows chip to be loaded once for support of sample processing anytime within an 8-hour window
• Flexible data display options - Results shown in your choice of virtual gel, electropherogram graph, or tabular formats
• Save Money! – Automated analysis procedure provides significant cost savings relative to the materials and labor required to run manual gels for RNA analysis
• Automated exporting of results
• Digital format facilitates review, export, and archiving of data
• Extended Workflow profile allows chip to be loaded once for support of sample processing anytime within an 8-hour window
• Flexible data display options - Results shown in your choice of virtual gel, electropherogram graph, or tabular formats
Demos
Our product can be demonstrated at our facility in Hopkinton, Massachussets or other Caliper offices around the world. Please contact your local Caliper Representative to arrange a demo.
Specifications
Size Range |
|
| 100 - 6,000 nucleotides | |
Quantitation Accuracy |
|
| +/- 30% | |
Linear Range |
|
| 50 – 500 ng/ul (high range) 5 -100 ng/ul (low range | |
Carryover |
|
| < 5% following 500 ng/ul sample | |
Analysis Time per Sample |
|
| 80 seconds |
