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IGF1R (h)

Cat#: 200-0466
Species: Human
RegionRecombinant cells
Enzyme FamilyTyrosine kinase (TK)
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)3E-3
Km (Peptide Substrate)>1E-4
ATP [M]3.00E-03
ReactionFluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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