LabChip® EZ Reader

Overview

The LabChip EZ Reader is a breakthrough benchtop instrument which facilitates kinase testing and other enzyme screening and profiling.  When combined with ProfilerPro kits, a low cost, turn-key solution of lab-bench kinase panel profiling is provided. LabChip assays are separations-based, so the quality of results exceeds what is achievable in homogenous, well-based assays.  The LabChip EZ Reader runs kinase and enzymatic assays with a 4-Sipper microfluidics chip.

Utilizing Caliper’s widely adopted mobility shift microfluidics assay technology, the LabChip EZ Reader analyzes kinase activity by ‘sipping’ stopped reactions from 96 or 384 well microtitre plates.  Caliper’s Mobility Shift Assay technology is designed for in-vitro enzyme assays aimed at the drug discovery process. It combines the advantages of capillary electrophoresis and microfluidics providing direct measurement of substrate and product, rapid assay development and real time kinetic reads. 

The EZ Reader is the perfect platform for running kinase profiling with the Caliper ProfilerPro Kits. ProfilerPro Kinase Profiling kits consist of matched pairs of 384 well kinase assay and plates complete with substrates.  The assay plates each contain 24 different kinases, pre-dispensed and frozen.  The substrate plates contain the appropriate fluorescent labeled peptides and ATP.

Using LabChip EZ Reader researchers can monitor enzyme reactions and kinase inhibitors over time, resulting in more detailed data resulting in using less reagents.  The advantages of microfluidics technology with real time kinetic analysis include:  simplified and accelerated assay development and optimization, calculation of Km and Ki values from initial rates, and determination of enzyme linearity.