LabChip® EZ Reader
The LabChip EZ Reader is a breakthrough benchtop instrument which facilitates kinase testing and other enzyme screening and profiling. When combined with ProfilerPro kits, a low cost, turn-key solution of lab-bench kinase panel profiling is provided. LabChip assays are separations-based, so the quality of results exceeds what is achievable in homogenous, well-based assays. The LabChip EZ Reader runs kinase and enzymatic assays with a 4-Sipper microfluidics chip.
Utilizing Caliper’s widely adopted mobility shift microfluidics assay technology, the LabChip EZ Reader analyzes kinase activity by ‘sipping’ stopped reactions from 96 or 384 well microtitre plates. Caliper’s Mobility Shift Assay technology is designed for in-vitro enzyme assays aimed at the drug discovery process. It combines the advantages of capillary electrophoresis and microfluidics providing direct measurement of substrate and product, rapid assay development and real time kinetic reads.
The EZ Reader is the perfect platform for running kinase profiling with the Caliper ProfilerPro Kits. ProfilerPro Kinase Profiling kits consist of matched pairs of 384 well kinase assay and plates complete with substrates. The assay plates each contain 24 different kinases, pre-dispensed and frozen. The substrate plates contain the appropriate fluorescent labeled peptides and ATP.
Using LabChip EZ Reader researchers can monitor enzyme reactions and kinase inhibitors over time, resulting in more detailed data resulting in using less reagents. The advantages of microfluidics technology with real time kinetic analysis include: simplified and accelerated assay development and optimization, calculation of Km and Ki values from initial rates, and determination of enzyme linearity.
Applications
• Other enzyme screening and profiling
• Primary screening
• Secondary screening
• Dose response (IC50) evaluations
• Mechanism of Action (MOA) studies
• Enzymatic assays for multiple target classes (including lipid and protein kinases)
Features
• Run real-time kinetic assays fir truer understanding of enzyme activity over time
• Automates sample analysis directly from 96- or 384-well plates
• Flexible workflow allowing samples to be run a few at a time, or batched for high throughput
• Automated exporting of results
• Supports ProfilerPro kinase profiling kits
• Multiple target classes including kinases, phosphatases, proteases, histome deacetylases, and phosphodieterase
Demos
Specifications
Power Requirements |
|
| 100/110/230 V AC, 50/60 Hz | |
Net Weight |
|
| 100 lbs (45.5 kg) | |
Dimensions |
|
| 18.28" (46.4 cm) x 18.60" (47.2 cm) x 25.57" (64.9 cm ) | |
Temperature Range |
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| 50–104 °F (10–40 °C) | |
Power Consumption |
|
| 460 VA (460 W) | |
Plate Formats |
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| 96- and 384-well | |
Excitation/Emission Wavelengths |
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| 470 and 530 nm | |
Humidity Range |
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| 5–85% relative, non-condensing |
Comments
Biogen-Idec, COG 2009
“Finally, the process that we have described reduces both experimentation cost and time while increasing throughput, making possible what–if experiments that, up to now, had been either prohibitively expensive or too complex to perform. This process results in better solutions, decreased development time and increased productivity.”
Vertex, COG ’09 & ’07 and Anal. Biochem., 2006:351 p122
“Microfluidic analysis of experiments moves the bottle neck from data analysis to experimental design.”
NCI-SAIC, COG 2009
“We achieved material cost savings of 50%+, and time savings of 75% per screen!”
Univ. of Wisconsin, Midwest LRIG 2007
“Caliper was willing to work with us to develop the 12 kb chip and provided tremendous support and interactions. We are very satisfied with the LabChip, will likely purchase 1-2 more and recommend this platform to our colleagues.”
Dow AgroSciences, COG 2007
