LabChip® EZ Reader II
The LabChip EZ Reader is a breakthrough benchtop instrument which facilitates kinase testing and other enzyme screening and profiling. When combined with ProfilerPro kits, a low cost, turn-key solution of lab-bench kinase panel profiling is provided. LabChip assays are separations-based, so the quality of results exceeds what is achievable in homogenous, well-based assays. The LabChip EZ Reader runs kinase and enzymatic assays with a 4-Sipper microfluidics chip.
Utilizing Caliper’s widely adopted mobility shift microfluidics assay technology, the LabChip EZ Reader analyzes kinase activity by ‘sipping’ stopped reactions from 96 or 384 well microtitre plates. Caliper’s Mobility Shift Assay technology is designed for in-vitro enzyme assays aimed at the drug discovery process. It combines the advantages of capillary electrophoresis and microfluidics providing direct measurement of substrate and product, rapid assay development and real time kinetic reads.
The EZ Reader is the perfect platform for running kinase profiling with the Caliper Profiler Pro Kits. Profiler Pro Kinase Profiling kits consist of matched pairs of 384 well kinase assay and substrate plates. The assay plates each contain 24 different kinases, pre-dispensed and frozen. The substrate plates contain the appropriate fluorescent labeled peptides and ATP.
The LabChip EZ Reader II provides the option to run assays with either a 4-Sipper or a 12-Sipper microfluidics chip, providing a complete solution for developing and running enzymatic assays.
Using LabChip EZ Reader II, researchers can monitor enzyme reactions over time, resulting in more detailed data resulting in using less reagents. The advantages of microfluidics technology with real time kinetic analysis include: simplified and accelerated assay development and optimization, calculation of Km and Ki values from initial rates, and determination of enzyme linearity.
Applications
• In-house kinase profiling and screening
• Other enzyme screening and profiling
• Primary screening
• Secondary screening
• Dose response (IC50) evaluations
• Mechanism of Action (MOA) studies
• Enzymatic assays for multiple target classes (including lipid and protein kinases)
• Other enzyme screening and profiling
• Primary screening
• Secondary screening
• Dose response (IC50) evaluations
• Mechanism of Action (MOA) studies
• Enzymatic assays for multiple target classes (including lipid and protein kinases)
Features
• Run real-time kinetic assays fir truer understanding of enzyme activity over time
• Automates sample analysis directly from 96- or 384-well plates
• Flexible workflow allowing samples to be run a few at a time, or batched for high throughput
• Automated exporting of results
• Supports ProfilerPro kinase profiling kits
• Multiple target classes including kinases, phosphatases, proteases, histome deacetylases, and phosphodieterases
• Automates sample analysis directly from 96- or 384-well plates
• Flexible workflow allowing samples to be run a few at a time, or batched for high throughput
• Automated exporting of results
• Supports ProfilerPro kinase profiling kits
• Multiple target classes including kinases, phosphatases, proteases, histome deacetylases, and phosphodieterases
Demos
Our product can be demonstrated at our facility in Hopkinton, Massachussetts or other Caliper Life Sciences offices worldwide. Please contact your local Caliper Representative to arrange a demo.
Specifications
Power Requirements |
|
| 100/110/230 VAC, 50/60 Hz | |
Dimensions |
|
| Height | 18.28" (46.4 cm) |
| Width | 18.60" (47.2 cm) |
| Depth | 25.57" (64.9 cm) |
Net Weight |
|
| 100 lbs. (45.5 kg) | |
Temperature Range |
|
| 10–40 °C (50–104 °F) | |
Power Consumption |
|
| 460 VA (460 W) | |
Plate Formats |
|
| 96- and 384-well | |
Excitation/Emission Wavelengths |
|
| 470 and 530 nm | |
Humidity Range |
|
| 5–85% relative, non-condensing | |
Comments
“LabChip enables us to perform experiments in an afternoon that would have required a month.”
Biogen-Idec, COG 2009
“Microfluidic analysis of experiments moves the bottle neck from data analysis to experimental design.”
NCI-SAIC, COG 2009
“We achieved material cost savings of 50%+, and time savings of 75% per screen!”
Univ. of Wisconsin, Midwest LRIG 2007
“Caliper was willing to work with us to develop the 12 kb chip and provided tremendous support and interactions. We are very satisfied with the LabChip, will likely purchase 1-2 more and recommend this platform to our colleagues.”
Dow AgroSciences, COG 2007
Biogen-Idec, COG 2009
“Microfluidic analysis of experiments moves the bottle neck from data analysis to experimental design.”
NCI-SAIC, COG 2009
“We achieved material cost savings of 50%+, and time savings of 75% per screen!”
Univ. of Wisconsin, Midwest LRIG 2007
“Caliper was willing to work with us to develop the 12 kb chip and provided tremendous support and interactions. We are very satisfied with the LabChip, will likely purchase 1-2 more and recommend this platform to our colleagues.”
Dow AgroSciences, COG 2007
