Living Image® 4.0

Acquire, Analyze & Discover:
3D Fluorescence and Bioluminescence

Advanced Acquisition & Analysis Tools for IVIS Instrument Systems

Living Image^® 4.0 software simplifies even the most advanced optical imaging protocol to facilitate success the first time and every time. The software’s new design creates an intuitive, seamless workflow to streamline acquisition through analysis. Living Image 4.0 software provides powerful new features such as wizard guidance for both acquisition parameter setup to post processing.  New quantitative 3D Fluorescence (FLIT4™) and Bioluminescence (DLIT4™) toolsets enable cell number or pmol determination of a fluorochrome. Co-registration with other imaging modalities or the digital “mouse atlas”  provides anatomical context to scientific data. The result is easy to use, high throughput and enables accurate quantitative data measurements. Living Image 4.0 software operates all IVIS^® imaging systems with analysis versions compatible with both Windows^® and Mac^® platforms.

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The Ultimate User Experience

Whether you are an experienced  imager or just beginning, Living Image 4.0  has the features to insure success. The imaging wizard will help guide you in choosing optimal acquisition settings to post processing parameters in a seamless manner. Monitor instrument usage amongst multiple users with Living Image 4.0’s new auditing tools. User preferences are automatically stored for each user.  

Enhanced 3D Fluorescence

Living Image 4.0 will open more solutions available to you in fluorescent optical imaging . Filter selections have been optimized for over for 40 different fluorescent proteins and dyes. Alternatively, characterize your dye of choice by performing emission and excitation scans to customize your acquisition settings. New fluorescent scalings are also available for determining efficiencies, cell number or pmol of fluorescent protein or dye. Experience a whole new spectral unmixing (SPUM) interface with tools that reduce cross-talk, allow real color rendering with photographic or X-ray overlay on composite SPUM images. Living Image 4.0 allows total flexibility in image adjustments to extract the best image from your detected signal.  

Quantiative 3D Imaging

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Living Image 4.0 introduces a whole new way of reconstructing and quantitating optical signals. Get clear signal location, 3D visualization and quantiation in terms of cell number or pmols of fluorescent compound. Harnessing IVIS Spectrum’s unique transillumination feature,  images are acquired at multiple transillumination points to scan a source intensity. The source is then reconstructed using Caliper’s unique reconstruction algorithms for Fluorescence Image Tomography for 4.0  (FLIT4). In combination with a surface topography scanning, the reconstruction can be co-registered with CT, MRI, PET or the organ atlas for anatomical context. 

Diffuse Light Imaging Tomography

3D Diffuse Luminescence Image Tomography (DLIT4), like fluorescence imaging, uses light scattering and absorption to determine depth and intensity of signal. However, images are acquired at several different wavelengths to improve localization of the source position. Living Image 4.0 enables you to monitor both DLIT and FLIT together with multi 3D overlays. Calibrate your cell lines or fluorochomes within well plates to determine absolute cell number or pmols.  Keep a library of calibrated models for future use!



or contact David Panzarella at +1-845-735-1921 or David.Panzarella@caliperls.com