MDA-MB-231-luc2-GFP Bioware® Ultra Green
MDA-MB-231-luc2-GFP is a luciferase and green fluorescent protein (GFP) expressing cell line. This cell line can be used in vivo to establish:
- Orthotopic Mamarry fat pad tumor models
- Subcutaneous tumor models
- Ex vivo cell isolation using fluorescent activated cell sorter (FACS)
- Post genomic analyses of isolated tumor cells.
- Immunohistochemistry detection of GFP positive cells
P/N: 128442
Designation: MDA-MB-231-luc2-GFP
Tissue: Human: Breast Cancer
Bioluminescence In Vitro: Approximately 1240 photons/sec/cell. Exact number will vary depending on imaging and culturing conditions.
Recommended Media: Eagle’s MEM ATCC (Catalog Number 30-2003)
Supplement the above with heat inactivated FBS at final concentration 10%.
In vivo Models Tested: Nu/nu (CR); orthotopic
In vitro Luciferase and GFP stability
500,000 cells were plated per well in black-walled 24-well plates. Cells were incubated for 37C for recovery overnight and luciferase assay was performed using an IVIS Spectrum. Each experiment was done in quadruplicates. The assays were repeated over four weeks period of time. Bioluminescence data was analyzed using the Living Image 4.0 software.
Growth curve of MDA-MB-231-luc2-GFP
1 x105 cells were plated on T25 flasks and the total numbers of cells were counted every 24 h using a Nexcelom automatic cell counter.
Subcutaneous Tumor Growth in a Nu/nu Mice
1 x 106 MDA-MB-231-luc2-GFP cells were injected orthotopically into the mammary fat pad in female nu/nu mice. Tumor growth was monitored by IVIS Spectrum at various time points for both luciferase and GFP expression. For luciferase expression, mice were imaged 10 minutes post i.p. injection of Luciferin at 150mg/kg at various time points. The image above shows tumor growth from representative mouse, while the chart shows tumor growth form luciferase and GFP reporters. GFP signal from the tumor was measured using the ex 465/ em 520 nm filters