Multispectral Microscopy Imaging Systems
Multi-label imaging without the cross-talk
Today’s biological imaging demands in-depth study of extremely complex systems, and often requires multi-label analysis. Caliper’s multispectral microscopy imaging systems enable quantitative evaluation of multiple fluorescent and brightfield molecular markers simultaneously even when co-localized in a single tissue section. The data for each marker is spectrally unmixed, generating clear and accurate images for each of the individual labels in a multi-labeled tissue section with no cross-talk.
Our microscopy imaging systems deliver the high quality data provided by flow-cytometry, but unlike this technique, the morphological context is preserved down to the sub-cellular level. In addition, autofluorescence is completely removed, increasing signal-to-noise by up to 100x. Low signal levels that were previously undetected can now be captured, and overall quantitation is vastly improved - particularly for solid, formalin-fixed, and paraffin-embedded tissues.
Caliper’s microscopy imaging systems are ideal for the following areas of research:
- Tissue biomarker discovery and validation
- Signaling pathway analysis in cancer research and drug development
- Microenvironment analysis, including assessment of tumor immunogenicity
- Tissue cytometry, including multiplexed per-cell measures of nuclear, cytoplasmic, and membranous protein expression
Brightfield immunohistochemistry with color re-mapping for legibility. Oral squamous carcinoma (FFPE, 20X) stained for CD34 (endothelium, Nova Red) and podoplanin (VIP, purple), and counterstained with hematoxylin. The spectral dataset was unmixed, the component planes re-colored (purple to green) and displayed as a composite image with improved contrast and legibility over the original.
If your research requires image analysis capabilities, Caliper’s inForm advanced analysis software incorporates powerful tissue segmentation algorithms with an intuitive learn-by-example interface. Users can easily train the program to automatically locate and analyze only targeted regions. Once trained, inForm can rapidly batch process large numbers of images – allowing analysis that might have taken days to be done in a matter of minutes.
Caliper’s microscopy imaging systems are an easy add-on to any microscope with a camera port, and enable simple image acquisition and high-contrast separation of multiple chromogens or fluorophores. Both high-resolution color and multispectral images are captured, and can be exported in standard image file formats. In addition, Nuance systems are compatible with Molecular Devices' MetaMorph™ software.
TRIO – Compact, affordable system for simple image capture of up to three chromogens or fluorophores.
Nuance EX – Microscopy system designed for simultaneous imaging of multiple fluorescent and NIR fluorescent molecular markers.
Nuance FX - Simultaneous imaging of multiple brightfield and fluorescent molecular markers.
Vectra - Automated, high-throughput multispectral imaging of multiple brightfield molecular markers.
There are the two Nuance systems:
| Naunce FX | Nuance EX | |
| Modality | Brightfield & Fluorescence | Fluorescence |
| Spectral Range (Brightfield) | 420-720 nm | 450-950 nm |
| Spectral Range (Fluorescence) | 420-720 nm | 450-950 nm |
| Brightfield Labels | Yes | |
| Fluorescent Labels | Yes | |
| Analysis Capability | Pixel-based ROI and colocalization, image comparison, batch processing | |
| inForm™ Compatibility | Yes | |
| Note: An F-mount macro lens is available for macroscopic applications. | ||
