Nuance™ FX

Flexible bandwidth multispectral microscopy

The Nuance FX multispectral imaging system is specifically tailored to meet the majority of all brightfield and fluorescence microscopy imaging applications.  

The system utilizes an optimized, high-throughput tunable filter distinctly matched to the bandwidths of common molecular markers. This solid-state filter is electronically switchable to maximize either sensitivity or throughput, enabling the Nuance FX to provide either chromogenic or fluorescence-based imaging. 

Nuance acquisition software incorporates easy to use tools for building custom spectral libraries, as well as powerful unmixing and analysis algorithms allowing spectral characterization and quantitation for each of the multi-labeled components in an image.  inForm advanced image analysis software is also an available option for rapid analysis of targeted tissues and batch processing of user-specified regions across multiple images.  Nuance FX is also compatible with Molecular Devices’ MetaMorph™ software.



Multiplexed imaging of quantum-dot stained tissue. Breast cancer (FFPE, 20X) stained for estrogen receptor (ER, Qdot 655 nm) and progesterone receptor (PR, Qdot 585 nm). Nuclei counterstained with DAPI, and tissue autofluorescence detected spectrally. Separate planes for each signal are shown. The second Nuance composite image displayed is in Brightfield Display Mode (BDM).



Nuance FX and EX Specifications

	Nuance FX	Nuance EX
Modality	Brightfield & Fluorescence	Fluorescence
Spectral Range (Brightfield)	420-720 nm	450-950 nm
Spectral Range (Fluorescence)	420-720 nm	450-950 nm
Brightfield Labels	Yes
Fluorescent Labels	Yes
Analysis Capability	Pixel-based ROI and colocalization, image comparison, batch processing
inForm™ Compatibility	Yes
Note: An F-mount macro lens is available for macroscopic applications.