HT Pico Protein Assay Kit
Automated Protein Sizing and Quantitation
The Caliper LabChip HT Pico Protein assay provides a fast and easy way to analyze protein samples between 14-200 kDa requiring sensitivity equivalent to Silver Stain levels. Protein detection for concentrations as low as 10 pg/ul, with a linear dynamic range of four orders of magnitude.
Performing protein sample analysis with the Pico Protein assay combines sample pre-labeling with automated steps for sample loading and quantitation. The LabChip GXII Electrophoresis System uses a single sipper microfluidic chip to aspirate protein samples directly from 96- or 384-well plates. The instrument optics detect the laser-induced fluorescent signal. System software automatically analyzes the data and provides the user with relative protein concentration, molecular size, and percent purity using ladder and marker calibration standards. Digital data results are immediately available for review or reporting in virtual gel, electropherogram graph, or table summary form.
Chips and Reagents sold separately:
• Protein Chip (P/N 760499)
• Pico Protein Reagent Kit (P/N 760498)
• Bulk Pico Protein Sample Buffer (P/N 760414)
Features
• Sizing Range – 14 to 200KDa
• Linear dynamic range of 4 logs
• Ability to resolve NGHC from HC under reducing conditions and MAb fragments (HHL) from intact antibody
• Automated exporting of results Digital data format facilitates review, export, and sharing of data
• Extended Workflow profile allows chip to be loaded once per day for support of various sample processing sessions
Specifications
Size Range |
|
| 14 kDa - 200 kDa | |
Quantitation Accuracy |
|
| 30% CV up to 120 kDa. Above 120 kDa, quantification is not specified. | |
Linear Range |
|
| Assay Ab Linear Concentration Range = 50 pg/µL – 500 ng/µL (4 logs) | |
Carryover |
|
| < 0.5% | |
Sizing Accuracy |
|
| +/- 20% | |
Resolution |
|
| +/- 10% difference in molecular weight | |
Sensitivity |
|
| 0.1% of the total protein |
