Kinase, Protein, PKA (h)
| Cat#: | 200-0440 |
| Species: | Human |
| Region | Recombinant cells |
| Enzyme Family | cAMP dependent protein kinase (AGC) |
| Substrate | Fluorescein-labeled peptide |
| Substrate [M] | 1.5E-6 |
| Km (ATP) | 4.6 E-06 |
| Km (Peptide Substrate) | 4.0 E-06 |
| ATP [M] | 4.6 E-6 |
| Reaction | Fluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP |
| Method | Fluorescence – electrophoretic mobility shift |
| Measurement | Separation of phosphorylated and unphosphorylated substrate |
| Reference Compound | Staurosporine IC50 = 2.08 E-9 M |
