EL1-Luc/EL1-TAg
Overview
EL1-Luc/EL1-TAg (Light Producing Transgenic Animal Model of Tag-Driven Pancreatic Cancer)
This mouse model can be used to study spontaneous pancreatic adenocarcinoma with bioluminescence as a read-out for the tumor burden. Two transgenes were made using a tissue-specific rat Elastase I promoter fragment of 220 base pairs to direct the expression of both, SV40 large T-antigen and modified firefly luciferase (Promega pGL3), in pancreatic exocrine cells. The transgenes were co-injected into the pronucleus of FVB zygotes, which resulted in cosegregation of luciferase and the oncogene in subsequent generations of mice. Characterization of transgenic mice indicates that the penetrance of bioluminescent tumor development in the pancreas is close to 100% and onset ranges from 60 to 100 days of age. Histopathological evaluation of the pancreas of these mice showed that the developing lesions are predominantly of acinar cell origin.
| Mouse Strain: | FVB/N-EL1-Luc/EL1-TAg |
| Common Name: | EL1-Luc/EL1-TAg |
| Genotype: | Hemizygous |
| Background Strain: | FVB/N |
| Coat Color: | Albino |
Applications
These transgenic mice develop fast growing and aggressive pancreatic adenocarcinoma that can be monitored through noninvasive bioluminescence imaging. This model can be used for non-invasive monitoring of spontaneous tumorogenesis in the pancreas, and for efficacy studies of therapeutic agents against pancreatic cancer.
