MDA-MB-231-luc-D3H2LN Bioware® Cell Line
Overview
The MDA-MB-231-luc-D3H2LN Bioware® is a luciferase expressing cell line that was derived from spontaneous lymph node metastasis of MDA-MB-231-luc-D3H1 cells implanted orthotopically into the mammary fat pad. This cell line has enhanced tumor take and metastatic potential compared to the parental MDA-MB-231-luc-D3H1. Selection Marker: Zeocin (Sensitivity: 0.05 mg/ml).
Tissue: Human: adenocarcinoma; mammary gland; pleura effusion
Bioluminescence In Vitro: Approx. 200 photons/second/cell, subject to imaging and culturing conditions
Parental Line Source (MDA-MB-231): American Type Culture Collection
In Vivo Models Tested:
Nu/nu (Harlan): mammary fat pad with metastasis to lymph nodes and lungs, intracardiac (colonization of various tissues); Nude-beige (CR): mammary fat pad with metastasis to lymph nodes and lungs
Applications
The MDA-MB-231-luc-D3H2LN Bioware® Cell Line can be used in vivo to establish an experimental metastasis model (intracardiac injection) and orthotopic mammary fat pad model with metastasis. Bioluminescent imaging detected tumor cells throughout the experiment.
