LoVo-6-luc-1 Bioware® Cell Line
Overview
The LoVo-6-luc-1 Bioware® Cell Line (P/N 119265) is a light producing cell line derived from LoVo human colorectal adenocarcinoma cells by stable transfection of the North American Firefly Luciferase gene expressed from the SV40 promoter. Selection Marker: Neomycin (G418 Sensitivity: 0.5 mg/ml).
Tissue: Human: adenocarcinoma, colorectal
Parental Line Source: American Type Culture Collection
Bioluminescence In Vitro: Approx. 2 photons/sec/cell depending on imaging and culturing conditions.
In Vivo Models Tested:
SCID-bg Male (CR): subcutaneous
SCID-bg Female (CR): subcutaneous, intraperitoneal, orthotopic (cecum injection)
Applications
The LoVo-6-luc-1 Bioware® Cell Line (P/N 119265) can be used in vivo to establish subcutaneous, intraperitoneal and cecum orthotopic tumor models in SCID-bg mice with >80% tumor take.
Visible bioluminescence signals were detected immediately after cell injection and a high tumor take rate was seen in all models. In the intraperitoneal and cecum-injected models, the onset of tumor spread was rapid and ex vivo imaging confirmed metastasis to multiple organs such as liver, lung, kidney, adrenal gland, spleen and ovary. Bioluminescent imaging detected tumor cells throughout the experiment; measurable tumors may be calipered within two weeks after cell injection in the subcutaneous model.
