PC-3M-luc-C6 Bioware® Cell Line
Overview
The PC-3M-luc-C6 Bioware® Cell Line is a light producing cell line derived from PC3M human adenocarcinoma cells by stable transfection of the North American Firefly Luciferase gene expressed from the SV40 promoter. This light producing cell line is available from Caliper Life Sciences, Alameda, CA. Selection Marker: Zeocin (Sensitivity: 0.2 mg/ml).
Tissue: Human: adenocarcinoma; prostate
Parental Line Source: MD Anderson Cancer Center, the University of Texas
Bioluminescence In Vitro: Approx. 150-250 photons/sec/cell, subject to imaging and culturing conditions.
In Vivo Models Tested:
Nu/nu (CR): subcutaneous, intravenous, intracardiac with multiple colonization sites (particularly bones), orthotopic
SCID-bg (CR): subcutaneous with multiple metastasis, intravenous with multiple colonization sites
Applications
The PC-3M-luc-C6 Bioware® Cell Line (P/N 119270) can be used in vivo to establish subcutaneous tumor models, subcutaneous tumor models with metastasis (SCID-bg mice), experimental metastasis models (intravenous and intracardiac), and an orthotopic prostate tumor model. Bioluminescent imaging detected tumor cells throughout the experiment. This cell line is particularly useful for model of prostate-to-bone metastasis after the intracardiac injection to male nu/nu mice.
