4T1-luc2 Bioware® Ultra
Overview
4T1-luc2 is a luciferase expressing cell line which was stably transfected with firefly luciferase gene (luc2). The cell line was established by transducing lentivirus containing luciferase 2 gene under the control of human ubiquitin C promoter.Designation: 4T1-luc2
Tissue: Mouse: mammary gland; adenocarcinoma
Source of Parental Line: ATCC (CRL-2539™)
Gene Transfer Vehicle: pGL4 luc2 Lentivirus
Bioluminescence In Vitro: Approximately 6700 photons/sec/cell. Exact number will vary depending on imaging and culturing conditions.
In vivo Models Tested: Nu/nu (CR); Subcutaneous
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Applications
• Mammary fat pad orthotopic model
• Subcutaneous tumor models
Non-invasive real-time monitoring of Metastasis by 4T1-luc2 cells
7.5 x 104 4T1-luc2 cells were implanted orthotopically in mammary fat pads of Balb/c mice. Primary tumors were resected 22 days post implant. Images shown above were taken 12 days post resection. Images were taken with an IVIS Lumina with exposure time of 60 sec. Luciferin was injected intraperitoneally at 150 mg/kg and mice were imaged 15 minutes post injection.
Subcutaneous tumor growth in a nu/nu mouse
Five 4T1-luc2 cells were injected subcutaneously into the dorsal region near the thigh of female nu/nu mouse. Bioluminescent signal could be detected in the animal way before it could be measured with a caliper. Tumor growth is monitored weekly by an IVIS Spectrum. Tumor was visible 28 days post implantation