pXen13 Plasmid

Overview

pXen13 (pSK luxCDABE)
This is a cloning vector carrying the original Photorhabdus luminescens luxCDABE operon for engineering bioluminescent Gram-negative bacteria. The bacterial
luciferase operon consists of luxC (751-2190), luxD (2205-3125), luxA (3177- 4256), luxB (4274-5254) and luxE (5364-6545) can be excised from the vector by restriction enzyme digestion.

General Purpose:  luxCDABE Vector
Plasmid Size:  8801 bp
Host Bacterium:  Escherichia coli  DH5a
Antibiotic Selection: Ampicillin at 100 μg/ml
Growth Medium:  LB
Growth Temperature: 37°C