HeLa-luc Bioware® Cell Line
The HeLa-luc Bioware® Cell Line (P/N 119272) is a light producing cell line derived from HeLa human cervical adenocarcinoma cells by stable transfection of the North American Firefly Luciferase gene expressed from the SV40 promoter. The in vitro growth of the cell line seem to be somewhat accelerated when compared to the parental cell line growth. Selection Marker: Zeocin (Sensitivity: 0.5 mg/ml).
Tissue: Human: adenocarcinoma, cervix
Parental Line Source: Stanford University (Dr. Chris Contag Laboratory)
Bioluminescence In Vitro: Approx. 250-400 photons/sec/cell, subject to imaging and culturing conditions
In Vivo Models Tested:
SCID-beige (CR): subcutaneous, intravenous lung colonization
Nu/nu (CR): subcutaneous (no lung colonization after intravenous injection)
NOTE: intravenous lung colonization model is not recommended for nu/nu mice.
Applications
The HeLa-luc Bioware® Cell Line (P/N 119272) can be used in vivo to establish subcutaneous and experimental lung metastasis models (after intravenous injection). The HeLa-luc cell line is useful as a standard for in vitro and in vivo bioluminescent studies. Bioluminescent imaging detected tumor cells throughout the experiment; tumors may be measured by caliper on week 3 after the implantation in the s.c. model.