XenoLight RediJect Coelenterazine h
Part Number: 760506RediJect Coelenterazine h is a bioluminescence substrate in a ready to use injectable format developed by the in vivo imaging leaders to fit your laboratory workflow. With Caliper’s Rediject Coelenterazine you can ensure reproducible results by minimizing variability in formulation, minimizing freeze/thaw and controlling batch to batch variation. Rediject Coelenterazine has been pre-formulated to fit common workflow patterns and optimize results for all your experiments with Renilla luciferase. The ready to use substrate formulation allows you to concentrate on animal handling and biology.
- Pre-formulated, batch controlled Coelenterazine h for in vivo use
- Save substantial time and effort by minimizing pre-imaging preparation steps
- Dispensed to image 5 animals per vial (10 vials/kit)
- In vivo imaging quality, validated on IVIS imaging systems
Applications
Coelenterazine h Optimized for In Vivo Imaging
Figure 1. Rediject Coelenterazine has been optimized for in vivo use with IVIS imaging systems. (A) 5 pmol BRET Qdot 700* were injected intravenously in a nu/nu mouse. 10 minutes post BRET Qdot injection 15 μg/mouse Coelenterazine h was injected intravenously. Chart shows peak signal was observed right after injection at ~ 22 seconds followed by rapid clearance. Images were taken with IVIS kinectic. (B) 1,2 and 4 pmol BRET Qdot 655 were injected subcutaneously in a nu/nu mouse. 10 minutes post BRET Qdot injection 10 μg/mouse Coelenterazine was injected intraperitoneally. Chart shows that peak signal was reached around 27 minutes post i.p. injection of Coelenterazine.
* BRET Qdot™ 700 were provided by Zymera Inc., 5941 Optical Court, San Jose, CA (http://zymera.com)
Qdot™ is a registered trademark of Life Technologies, 5791 Van Allen Way, Carlsbad, CA 92008
Features
Color and Form: Yellow colored solution (Coelenterazine h in Propylene glycol/citrate)
Concentration: 150 μg/mL
Volume per vial: 10 sterile vials each containing 1.2 mL of 150 μg/mL Coelenterazine h
Storage and Handling: Store at ≤ -70 °C. Just before your experiment, thaw required number of vials in a 37 °C water bath, vortex and it is ready to use. Repeated freeze thaw is not recommended. Rediject Coelenterazine supports intravenous (i.v.) or intraperitoneal (i.p.) injection.
For intravenous injections, recommended dose is 15 μg/mouse (100 μL/mouse). Load a 1 mL syringe di¬rectly from the vial and inject using a 25 gauge needle or higher. Image the mice as soon as possible for peak signal with intravenous injection. For intraperitoneal injection, recommended dose is 30 μg/mouse (200 μL/mouse)
Concentration: 150 μg/mL
Volume per vial: 10 sterile vials each containing 1.2 mL of 150 μg/mL Coelenterazine h
Storage and Handling: Store at ≤ -70 °C. Just before your experiment, thaw required number of vials in a 37 °C water bath, vortex and it is ready to use. Repeated freeze thaw is not recommended. Rediject Coelenterazine supports intravenous (i.v.) or intraperitoneal (i.p.) injection.
For intravenous injections, recommended dose is 15 μg/mouse (100 μL/mouse). Load a 1 mL syringe di¬rectly from the vial and inject using a 25 gauge needle or higher. Image the mice as soon as possible for peak signal with intravenous injection. For intraperitoneal injection, recommended dose is 30 μg/mouse (200 μL/mouse)
