XenoLight RediJect D-Luciferin Ultra
Part Number: 760505RediJect D-Luciferin Ultra (K+ salt) is a novel bioluminescence substrate in a ready to use pre-formulated injectable format developed by the in vivo imaging leaders to fit your laboratory workflow. Rediject D-Luciferin Ultra is pre-formulated with a fluorescent marker to validate your substrate injection. With Caliper’s Rediject D-Luciferin Ultra you can ensure reproducible results by minimizing variability due to formulation and substrate injection, minimizing freeze/thaw and controlling batch to batch variation.
Rediject D-Luciferin Ultra is pre-formulated with a rapidly clearing fluorescent dye to validate the integrity of your substrate injection, so with a quick fluorescent image on your IVIS system you know immediately if an injection failed and you can normalize your bioluminescence results. With Rediject D-Luciferin Ultra, you can have extra confidence in your data quality.
- Pre-formulated, batch controlled D-Luciferin for in vivo use
- Instantly validate your injection quality and minimize data variability
- Save substantial time and effort by minimizing pre-imaging preparation steps
- Dispensed to image 5 animals per vial* (10 vials/kit)
- In vivo imaging quality, validated on IVIS imaging systems
Applications
Instantly Validate Substrate Injection Quality
Figure 1. Mice (n=10) were implanted with LL/2-luc tumors subcutaneously and imaged on Day 11 and 12 post implant. Mice were injected by an intraperitoneal route (i.p.) with 150 μL Rediject Luciferin Ultra solution (150 mg/kg). Just before taking the BLI images, fluorescent imaging was performed on the dorsal side. Fluorescent images were quantified by placing the region of interest away from the abdominal region where the substrate is i.p. injected to get a better read out of the systemic distribution of the substrate. For dorsal images, the ROI is drawn around the scruff area (back of neck) for quantification of the reference fluorescence signal, while for ventral images the ROI is drawn around the thoracic region. Fluorescent signal is measured in efficiency units. In this study mouse #14 and 7 showed a decline or no bioluminescent signal due to incorrect substrate injection. These missed injections were instantly picked up by a drop in Fluorescent signal of greater than 30% in both instances when compared to the average Fluorescent signal. In this study any mouse that had greater than a 30% decrease in Fluorescent signal from the average needed to be reimaged.
Figure 1. Mice (n=10) were implanted with LL/2-luc tumors subcutaneously and imaged on Day 11 and 12 post implant. Mice were injected by an intraperitoneal route (i.p.) with 150 μL Rediject Luciferin Ultra solution (150 mg/kg). Just before taking the BLI images, fluorescent imaging was performed on the dorsal side. Fluorescent images were quantified by placing the region of interest away from the abdominal region where the substrate is i.p. injected to get a better read out of the systemic distribution of the substrate. For dorsal images, the ROI is drawn around the scruff area (back of neck) for quantification of the reference fluorescence signal, while for ventral images the ROI is drawn around the thoracic region. Fluorescent signal is measured in efficiency units. In this study mouse #14 and 7 showed a decline or no bioluminescent signal due to incorrect substrate injection. These missed injections were instantly picked up by a drop in Fluorescent signal of greater than 30% in both instances when compared to the average Fluorescent signal. In this study any mouse that had greater than a 30% decrease in Fluorescent signal from the average needed to be reimaged.
Features
Color and Form: Yellow colored solution (D-Luciferin Potassium salt in PBS)
Concentration: 30 mg/mL
Volume per vial: 10 sterile vials each containing 850 μL of 30 mg/ml D-Luciferin
Storage and Handling: Store at ≤ -70 °C. Just before your experiment, thaw required number of vials in a 37 °C water bath, vortex and it is ready to use. Repeated freeze thaw is not recommended. Rediject D-Luciferin supports intravenous, subcutaneous or intraperitoneal injection.
For in vivo imaging studies, we recommend intraperitoneal injection at 150 mg/kg (150 μL /mouse*). Load a 1 mL syringe directly from the vial and inject using a 25 gauge needle. For Fluorescence imaging, select exposure time between 1 and 5 seconds and image using 745 nm excitation and 800 nm emission filter set (ICG filter set for IVIS Lumina). Select an ROI on the scruff of the neck on the dorsal side to evaluate the accuracy of your intraperitoneal injection. For ventral imaging, ROI should be drawn around the thoracic region.
* Calculations based on a 30g mouse
Concentration: 30 mg/mL
Volume per vial: 10 sterile vials each containing 850 μL of 30 mg/ml D-Luciferin
Storage and Handling: Store at ≤ -70 °C. Just before your experiment, thaw required number of vials in a 37 °C water bath, vortex and it is ready to use. Repeated freeze thaw is not recommended. Rediject D-Luciferin supports intravenous, subcutaneous or intraperitoneal injection.
For in vivo imaging studies, we recommend intraperitoneal injection at 150 mg/kg (150 μL /mouse*). Load a 1 mL syringe directly from the vial and inject using a 25 gauge needle. For Fluorescence imaging, select exposure time between 1 and 5 seconds and image using 745 nm excitation and 800 nm emission filter set (ICG filter set for IVIS Lumina). Select an ROI on the scruff of the neck on the dorsal side to evaluate the accuracy of your intraperitoneal injection. For ventral imaging, ROI should be drawn around the thoracic region.
* Calculations based on a 30g mouse