XenoLight RediJect Inflammation Probe
XenoLight RediJect Inflammation probe is a chemiluminescent reagent for monitoring Inflammation. This probe is offered in a ready-to-use format and can be conveniently applied to study myeloperoxidase (MPO) activity of activated phagocytes. The RediJect Inflammation Probe will allow for longitudinal tracking of MPO level and inflammation status, in vivo, in a variety of disease models, including rheumatoid arthritis, acute septic shock, contact hypersensitivity reaction, and neutrophil rich cancers.
- Novel ready-to-use probe to monitor phagocyte mediated inflammation non-invasively
- Dispensed to image 5 animals (explorer kit) or 20 animals (standard kit)*
- Includes a fluorescent tracer to validate substrate injection
- Improved sensitivity due to the chemiluminescent read-out
- In vivo imaging quality, validated on IVIS imaging systems
XenoLight RediJect Inflammation Probe (Standard kit)
Detection of Inflammation in a Rheumatoid Arthritis (RA) Model
DBA1 mice were induced to develop RA with Atherogen monoclonal antibody cocktail (Chondrex, Inc). Mice were monitored for arthritis development by conventional visual scoring method (red curve, bottom panel) and by quantifying the chemiluminescent signal from the diseased tissues (blue curve, bottom panel). On day11, the diseased mice showed severe inflammation in the extremities, which correlated with an increase of chemiluminescent signal at the knee joints, front and rear paws (Top left) when compared to control mice (top right panel). Images were taken 10 minutes post i.p. injection of the probe with IVIS Spectrum (5 minutes exposure time).
Applications
- Rheumatoid arthritis,
- Acute septic shock
- Contact hypersensitivity reaction
- Neutrophil rich cancers
Features
Concentration: 40 mg/ml
Shipping condition: The kit will be shipped in cold gel packs to avoid temperature variations
Volume per vial: Explorer kit: 1 sterile amber vial containing 850 ul of 40 mg/ml probe
Standard kit: 4 sterile amber vials containing 850 ul of 40 mg/ml probe
Storage and Handling: Store at ≤ -20°C, thaw the vial in a 37°C water bath just before experiment, vortex and it is ready-to-use. Repeated freeze thaw is not recommended.
For in vivo imaging studies, we recommend intraperitoneal (i.p.) injection at 200 mg/kg (150 ul /mouse*). Load a 1ml syringe directly from the vial and inject using a 25 gauge needle. Best time to image is 10 minutes post i.p. injection of the probe. We recommend exposure time of 5 minutes for better sensitivity. For Fluorescence imaging, select exposure time between 1 and 5 seconds and image using 745 nm excitation and 800 nm emission filter set (ICG filter set for IVIS Lumina). Select an ROI on the scruff of the neck on the dorsal side to evaluate the accuracy of your intraperitoneal injection. For ventral imaging, ROI should be drawn around the thoracic region.
* Calculations based on a 30g mouse