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HT DNA 5K LabChip® GX/GXII Assay Kit

Overview

The HT DNA 5K LabChip kit for use on the LabChip GX & GXII systems provides a fast and easy way to analyze a DNA samples ranging from 100 to 5000 base pairs.  Performing DNA sample analysis with the DNA 5K assay saves time and money by automating steps for analyzing size and purity, and replaces agarose slab gels, and imaging.

The LabChip GX family of Electrophoresis Systems uses a single sipper microfluidic chip to aspirate dsDNA samples directly from 96- or 384-well plates. The microfluidics chip technology automatically mixes in an intercolating dye, electrophoretically separates, and analyzes the DNA sample.  The instrument optics detect the laser-induced fluorescent signal.  System software automatically analyzes the data and determines fragment size and concentration using ladder and marker calibration standards.  Digital data results are immediately available for review or reporting in virtual gel, electropherogram graph, or table summary form.




Applications

DNA Amplification/Sequencing

Features

• Save Time! – High throughput provides complete automated analysis of DNA fragments in under 30 seconds
• Save Money! – Automated analysis procedure provides significant cost savings relative to the materials and labor required to run manual gels for DNA analysis
• Increase Collaboration Opportunites! – Expand your opportunities to collaborate on projects with scientists around the world by utilizing the digital standard of the LabChip 90
• Automated exporting of results
• Digital format facilitates review, export, and archiving of data
• Extended Workflow profile allows chip to be loaded once for support of sample processing anytime within an 8-hour window
• Flexible data display options - Results shown in your choice of virtual gel, electropherogram graph, or tabular formats

Demos

Our product can be demonstrated at our facility in Hopkinton, Massachusetts or other Caliper Life Sciences offices worldwide.  Please contact your local Caliper Representative to arrange a demo.

Specifications

Size Range

100 - 5,000 bp

Linear Range

0.25 – 80 ng/ul

Analysis Time per Sample

30 seconds

Sizing Accuracy

+/- 10%

Resolution

10% - 20% difference in bp dependent upon position in the sizing range, approx comparable to a single % agarose gel

Sensitivity

0.25 ng/ul per fragment