LabChip XT DNA 300 Assay Kit
The LabChip XT DNA 300 Assay Kit is for use on the LabChip XT/XTe systems. It provides a fast and easy way to analyze and fractionate DNA samples ranging from 25 to 500 base pairs with improved collection efficiency (> 50%) and superior sensitivity, detecting less than 25ng of sheared sample. The high resolution separation makes it ideal for size selection of small RNA libraries from neighboring adaptor dimers.
LabChip XT DNA 300 Assay Kit (P/N 760601) consists of:
Box 1
• XT DNA 500 Ladder (yellow vial)
• XT DNA 6x Sample Buffer (green vial)
• XT DNA Collection Buffer (red vial)
• XT DNA Dye (blue vial)
• XT DNA Stacking Buffer (pink vial)
Box 2
• LabChip XT DNA 300 Chip Kit (5 Chips)
Features
• No post purification- Sample is delivered in a sequencing compatible buffer
• Fast and reproducible size selection
• Fast separation time –up to 300 bp in 45 minutes or less
• Up to 4 samples processed simultaneously per chip
• Completely independent channels minimizes potential for cross contamination
Specifications
Size Range |
|
| 25 - 500bp | |
Minimum Size Distribution |
|
| As expressed by %CV: 5% at 300bp | |
Maximum Load Capacity |
|
| 1 μg of sheared genomic DNA, 50ng per fragment | |
Minimum Load for Optical Detection |
|
| 25ng sheared genomic DNA, 0.5ng fragment | |
Sample Recovery |
|
| 50% - 90% | |
Time to Max bp |
|
| < 45 minutes (300bp) |
