Vectra™
Intelligent multispectral slide analysis system
Vectra’s automated, high-throughput imaging capabilities make it the most advanced system available for extracting proteomic and morphometric information from intact tissue sections. Combining three key components - automated slide-handling, multispectral imaging technology and pattern recognition-based image analysis – the system is a powerful tool for today’s pre-and clinical studies. Vectra is well suited for automated applications in:
- Tissue biomarker discovery and validation
- Signaling pathway analysis in cancer research and drug development
- Microenvironment analysis, including assessment of tumor immunogenicity
- Tissue cytometry, including multiplexed per-cell measures of nuclear, cytoplasmic, and membranous protein expression
The system accurately measures protein expressions and morphometric characteristics on whole slides or in distinct tissue regions of interest. Imaged tissue sections can be labeled with immunofluorescent (IF) or immunohistochemical (IHC) stains, or with conventional stains such as H&E and trichrome. When using IF or IHC stains, one or multiple proteins can be measured on a per tissue, per cell, or per cell compartment (e.g. nuclear, cytoplasmic) basis - even when signals are spectrally similar, in the same cell compartment or obscured by autofluorescence.
Powered by inForm™ software, Vectra automates quantitative data acquisition and then streamlines data extraction on up to 200 slides in a single batch run. inForm combines powerful tissue segmentation algorithms with an intuitive learn-by-example interface that users can train to identify specific tissue segments. Once trained, the program locates the specified regions automatically in all images. Automated acquisition in a batch run involves 4x whole slide scans followed by 20x high-magnification imaging for regions of interest only. This approach greatly reduces acquisition and analysis time and makes the most efficient use of hard disk space.
Click here to see how Vectra can help you, for a limited time you can send us your slides for a free analysis!
Vectra automatically performs a low-power 4x scan of your tissue microarrays, extracting only relevant, interesting high-power 20x fields. Intelligent imaging provides a more efficient and faster alternative to whole-slide scanning. The period of time to perform a study can be reduced from months or weeks to days or even hours.
| Vectra single-slide | Vectra 200-slide | |
| Automation | Single-slide | 200-slides without supervision |
| Stage Adjustment | Manual | Automated |
| Tissue Formats | Tissue microarrays (TMAs) and whole tissue sections | |
| Wavelength Range | 420 - 720 nm | |
| Throughput (based on intelligent field selection) | TMA: 3 cores/min Sections: 5-20 min per slide | |
| Modality | Brightfield or fluorescence (multispectral or color) | |
| Multiplexing capability | Separates markers, even overlapping, incl. autofluorescence | |
| Image Analysis (inForm™ software) | Intuitive learn-by-example interface, auto-segment and quantitate tissue structures, cells, sub-cellular signatures | |
| Magnification | 4x and 20x | |
| Barcode Reader | 1D/2D optional; reads MicroPDF, PDF 41, RSS, QR, Data Matrix, and other (1D) | |
| File Format | .im3 for multispectral (CRi); 24-bit TIFF for RGB | |
| Operating System | Windows™ XP Pro, SP-2 or later | |
| Computer | Quad Core Xeon, 1TB mirrored HDD minimum | |
| Required Bench Space | 6 x 2.5 ft | |
| Power Supply | 110/220 VAC | |
| CE Compliant | Yes | Yes |
| Note: An F-mount macro lens is available for macroscopic applications. | ||
