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Lab Automation and Liquid Handling Application Notes


 Drug Discovery

Compound Distribution form Matrix Screenmates Tubes using the Caliper Sciclone G3 (PDF)

Integration of an automated Workstation for Cell-Based Assays on Permeable Support Systems (PDF)

Improvements in High Content Cell-Based Assays using the Caliper Sciclone G3 and various Corning Microplates (PDF)

Miniaturization and Automation of a Luciferase Reporter Gene Assay (PDF)
 

PhyNexus PhyTips® and Caliper Automation

Optimization Strategies for High Performance Purification and Analysis of Recombinant Proteins with Micro Volume PhyTip Columns and Caliper Life Sciences Automation (PDF)

PhyTip Purification and Enrichment for Immobilized Metal Affinity Chromatography (IMAC) Resins (5 uL) with Caliper Life Sciences Automation (PDF)

PhyTip Purification and Enrichment for Immobilized Metal Affinity Chromatography (IMAC) Resins (20 uL) with Caliper Life Sciences Automation (PDF)
 

 Genomics

Zephyr Method Notes

Automation of the EdgeBio ExcelaPure 96-Well UF PCR Purification Kit on the Zephyr Molecular Biology Workstation (ZH-MN-001) (PDF) 

Automation of the Promega Wizard SV 96 Genomic DNA Purification System on the Zephyr Molecular Biology Workstation (ZH-MN-002) (PDF)

Automation of the Millipore MultiScreenHTS PCR96 Filter Plate on the Zephyr Molecular Biology Workstation (ZH-MN-003) (PDF)

Automation of the Millipore Montage SEQ96 Sequencing Reaction Cleanup Kit on the Zephyr Molecular Biology Workstation (ZH-MN-004) (PDF)

Automation of the Promega SV 96 Total RNA Isolation System on the Zephyr Molecular Biology Workstation (ZH-MN-005) (PDF)

Automation of the Promega Wizard SV 96 Plasmid DNA Purification System on the Zephyr Molecular Biology Workstation (ZH-MN-006) (PDF)

Automation of the Macherey-Nagel NucleoSpin 96 Tissue Kit on the Zephyr Molecular Biology Workstation (ZH-MN-007) (PDF)

Automation of the Macherey-Nagel NucleoFast 96 PCR Cleanup Kit on the Zephyr Molecular Biology Workstation (ZH-MN-008) (PDF)

Automation of the Qiagen QIAprep 96 Turbo Miniprep Kit on the Zephyr Molecular Biology Workstation (ZH-MN-009) (PDF)

Automation of the Macherey-Nagel NucleoSpin 96 Total RNA Isolation Kit on the Zephyr Molecular Biology Workstation (ZH-MN-010) (PDF)

Automation of the Qiagen QIAquick 96 PCR Purification Kit on the Zephyr Molecular Biology Workstation (ZH-MN-011) (PDF)

Automation of the Macherey-Nagel NucleoSpin Robot-96 Plasmid Kit on the Zephyr Molecular Biology Workstation (ZH-MN-012) (PDF)

Automation of the Agencourt CleanSEQ Sequencing Reaction Cleanup Kit on the Zephyr Molecular Biology Workstation (ZH-MN-013) (PDF)
Automation of the Agencourt Ampure XP SPRI Bead PCR Purification Protocol on the Zephyr Molecular biology Workstation (ZH-MN-014) (PDF)
 

Sciclone G3 (ALH 3000)

Preparing Agilent 2100 LabChips for RNA analysis using the Caliper Sciclone G3 (SC-AP-706) (PDF)

Efficient, Automated RNA Isolation Using Ambion MagMAX-96 Total RNA Isolation Kit on the Sciclone G3 (SC-AP-710) (PDF)

Automation of the Ambion MagMAX-96 Viral RNA Isolation Kit on the Caliper Life Sciences ALH 3000 Workstation (SC-AP-711) (PDF)

Automated Antisense RNA Synthesis Using the Ambion MessageAmp II-96 aRNA Amplification Kit (SC-AP-712 (PDF)

High Throughput Plasmid DNA Preparation Using a Megnetic Bead Kit on an Automated Workstation (SC-AP-713) (PDF)

Scalable automation of the TempliPhi™ DNA Sequencing Template Amplification and DYEnamic™ ET Terminator Sequencing Kits on the Sciclone G3  (SC-AP-714) (PDF)

Sciclone ALH Carryover Testing Using the Ultrasonic Wash Station and a 384-Cannula Array (SC-AP-721) (PDF)

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone G3 (SC-AP-722) (PDF)
 

 Proteomics

A Novel Approach to Automated High-Throughput Protein Enrichment and Characterization (LCGX-AP-401) (PDF)

High Throughput Purification and Characterization of a Protein Variant Library (LCGX-AP-405) (PDF)

Protein Crystallization:  Sitting Drop Plate Screen Set-up using Corning's 96 Well Crystallography Plate and Caliper's Sciclone G3 (AP20) (PDF)
 

 Performance Validation

Evaluation of Second Generation Disposable Tip Accuracy and Precision for the Sciclone G3 using the Artel MVS Calibration Kit (SC-AP-700) (PDF)

Dry Dispensing with the Caliper Sciclone G3 using Disposable Tips (SC-AP-705) (PDF)

Liquid Level Detection with the Caliper Sciclone G3 (SC-AP-701) (PDF)

Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option (SC-AP-715) (PDF)

Prolonged Mechanical Stress Precision Testing on a Sciclone Low Volume Head (SC-AP-716) (PDF)

Dispensing 0.5 uL to "Dry" 384 Plates with the Sciclone (SC-AP-717) (PDF)

Sciclone Bulk Dispense Precision Testing (SC-AP-718) (PDF)

384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler (SC-AP-719) (PDF)

96 Well Pipetting Precision Testing Using the Sciclone G3 with a 384 Fixed-Cannula Array (SC-AP-720) (PDF)

Sciclone G3 Liquid Handler:  Functional Characterization of the High Volume Head: Plate-to-Plate Precision Testing (SC-AP-723) (PDF)
 

 Method Writing Tips

ALH 3000 Software v3.5: Using Excel Instructions and a Plate Normalization Example (SC-AP-702) (PDF)

Timing incubations using VBA in Caliper Sciclone Software v3.5 (SC-AP-703) (PDF)
 
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