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Microfluidic Publications

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2010

Development of a capillary gel electrophoresis method for monitoring disulfide isomer heterogeneity in IgG2 antibodies
Electrophoresis
Volume: 31. Issue: 3. Pages: 448-458. Year: 2010. Lacher NA

Capillary microfluidic electrophoretic mobility shift assays: application to enzymatic assays in drug discovery
Expert Opinion in Drug Discovery
Volume: 5. Issue: 1. Pages: 51-63. Year: 2010. Perrin, D

Development, validation, and implementation of capillary gel electrophoresis as a replacement for SDSPAGE for purity analysis of IgG2 mAbs
J. Sep. Sci.
Volume: 33. Issue: . Pages: 1-10. Year: 2010. Nathan A. Lacher

2009

Kinetic mechanism and inhibitor characterization of WNK1 kinase
Biochemistry
Volume: 48. Issue: 43. Pages: 10255-10266. Year: 2009. Yagi YI

Discovery of novel sphingosine kinase 1 inhibitors
Bioorganic & Medicinal Chemistry Letters
Volume: 19. Issue: . Pages: 6119–6121. Year: 2009. Yibin Xiang

PD-1 Blockade in Rhesus Macaques: Impact on Chronic Infection and Prophylactic Vaccination
The Journal of Immunology
Volume: 182. Issue: . Pages: 980-987. Year: 2009. Adam C. Finnefrock

Identification of Death-Associated Protein Kinases Inhibitors Using Structure-Based Virtual
Jrnl. Medicinal Chemistry
Volume: 52. Issue: . Pages: 7323–7327. Year: 2009. Masako Okamoto

Measuring and interpreting the selectivity of protein kinase inhibitors
J Chem Bio
Volume: 2. Issue: . Pages: 131-151. Year: 2009. Lynette A. Smyth & Ian Collins

A Predictive High-Throughput Scale-Down Model of Monoclonal Antibody Production in CHO Cells
Biotechnology and Bioengineering
Volume: 104. Issue: 6. Pages: 1107-1120. Year: 2009. Legmann R

A High Throughput Dimer Screening Assay for Monoclonal Antibodies Using Chemical Cross-linking and Microchip Electrophoresis
Jrnl. Chromatography B
Volume: . Issue: . Pages: . Year: 2009. Xiaoyu Chen and Gregory C. Flynn

The effect of Fc glycan forms on human IgG2 antibody clearance in humans
Glycobiology
Volume: 19. Issue: 3. Pages: 240-249. Year: 2009. Xiaoyu Chen, Y Diana Liu, and Gregory C Flynn

Discovery of OSI-906: a selective and orally efficacious dual inhibitor of the IGF-1 receptor and insulin receptor
Future Med Chem
Volume: 1. Issue: 6. Pages: 1153-1171. Year: 2009. Mulvihill, M.

Multiplex Assay for Simultaneously Typing and Subtyping Influenza
Jrnl. of Clinical Microbiology
Volume: 47. Issue: 2. Pages: 390-396. Year: 2009. Ying Huang, Huong Tang,1 Stuart Duffy, Yuwen Hong,1 Sylvia Norman,Madhu Ghosh

A Novel, Highly Selective, Tight Binding IκB Kinase-2 (IKK-2) Inhibitor : A Tool to Correlate IKK-2 Activity to the Fate and Functions of the Components of the Nuclear Factor-κB Pathway in Arthritis-Relevant Cells and Animal Models
JPET
Volume: 329. Issue: 1. Pages: 14-25. Year: 2009. Gabriel Mbalaviele

High-Throughput Biochemical Kinase Selectivity Assays: Panel
Jrnl Biomol Screening
Volume: 14. Issue: 1. Pages: 31-42. Year: 2008. Card, A.

Designing experiments for high-throughput protein expression
Methods Mol Biol
Volume: 498. Issue: . Pages: 19-29. Year: 2009. Chambers SP, Swalley SE.

E. coli and insect cell expression, automated purification and quantitative analysis.
Methods Mol Biol
Volume: 498. Issue: . Pages: 143-156. Year: 2009. Chambers SP, Fulghum JR, Austen DA, Lu F, Swalley SE.

2008

Small-scale, semi-automated purification of eukaryotic proteins for structure determination.
J Struct Funct Genomics
Volume: 8. Issue: 4. Pages: 153-166. Year: 2008. Frederick RO, Bergeman L, Blommel PG, Bailey LJ, McCoy JG, Song J, Meske L, Bingman CA, Riters M, Dillon NA, Kunert J, Yoon JW, Lim A, Cassidy M, Bunge J, Aceti DJ, Primm JG, Markley JL, Phillips GN Jr, Fox BG.

Wheat germ cell-free translation, purification, and assembly of a functional
Protein Expr Purif.
Volume: 62. Issue: 2. Pages: 171-178. Year: 2008. Michael A. Goren , Brian G. Fox

Microchip assays for screening monoclonal antibody product quality.
Electrophoresis
Volume: 29. Issue: 24. Pages: 4993-5002. Year: 2008. Chen X, Tang K, Lee M, Flynn GC.

Human IgG2 Antibody Disulfide Rearrangement in Vivo
J Biol Chem.
Volume: 283. Issue: 43. Pages: 29266-72. Year: 2008. Liu YD, Chen X, Enk JZ, Plant M, Dillon TM, Flynn GC.

Managing the Analytical Life-Cycle for Biotechnology Products
BioProcess International
Volume: . Issue: . Pages: 12-19. Year: 2008. Izydor Apostol

Mocr: a novel fusion tag for enhancing solubility that is compatible with structural biology applications.
Protein Expr Purif.
Volume: 63. Issue: 1. Pages: 40-49. Year: 2008. DelProposto J, Majmudar CY, Smith JL, Brown WC.

High-throughput assays for sirtuin enzymes: a microfluidic mobility shift assay and a bioluminescence assay.
Anal Biochem
Volume: 378. Issue: 1. Pages: 53-59. Year: 2008. Liu, Y.

2007

Development of a protease production platform for structure-based drug design.
Curr Protein Pept Sci.
Volume: 8. Issue: 5. Pages: 439-445. Year: 2007. Fox T, Brennan D, Austen DA, Swalley SE, Coll JT, Raybuck SA, Chambers SP.

A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis
PLoS ONE
Volume: . Issue: . Pages: e577. Year: 2007. Tal Murthy, Andreas Rolfs, Yanhui Hu, Zhenwei Shi, Jacob Raphael, Donna Moreira, Fontina Kelley, Seamus McCarron, Daniel Jepson, Elena Taycher, Dongmei Zuo, Stephanie E. Mohr, Mauricio Fernandez, Leonardo Brizuela, Joshua LaBaer

Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition
Biotechnol Prog.
Volume: 23. Issue: 3. Pages: 585-598. Year: 2007. Blommel PG, Becker KJ, Duvnjak P, Fox BG.

Two Simple and Generic Antibody-Independent Kinase Assays
Journal of Biomolecular Screening
Volume: . Issue: . Pages: 409-416. Year: 2004. Antje Pommereau, Everard Pap, Aimo Kannt

Functional Protein Microarrays in Drug Discovery
Volume: 8. Issue: . Pages: . Year: 2007. Paul F. Predki

2006

A Microfluidics-Based Mobility Shift Assay to Discover New Tyrosine Phosphatase Inhibitors
Journal of Biomolecular Screening
Volume: . Issue: . Pages: 996-1004. Year: 2006. Dominique Perrin, Christèle Frèmaux, Dominique Besson, Wolfgang Hb Sauer, and Alexander Scheer

Screening factors effecting a response in soluble protein expression: formalized approach using design of experiments.
Anal Biochem
Volume: 351. Issue: 1. Pages: 122-127. Year: 2006. Swalley SE, Fulghum JR, Chambers SP.

Assay Development and Screening of a Serine/Threonine Kinase in an On-Chip Mode Using Caliper Nanofluidics Technology
Biomolecular Screening
Volume: . Issue: . Pages: 359-368. Year: 2006. Dominique Perrin, Christèle Frèmaux, and Alexander Scheer

A High-Throughput Microfluidic Assay for SH2 Domain-Containing Inositol 5-Phosphatase 2
ASSAY and Drug Development Technologies
Volume: 4. Issue: 2. Pages: 175-183. Year: 2006. Todd Rowe, Clarence Hale, Aileen Zhou, Robert J.M. Kurzeja,Robert J.M. Kurzeja, Arisha Ali, Anthony Menjares, Minghan Wang, and John D. McCarter

Overcoming the Hurdle of Fluorescent Compounds in Kinase Screening: A Case Study
ASSAY and Drug Development Technologies
Volume: 4. Issue: 2. Pages: 185-196. Year: 2006. Dominique Perrin, Thierry Martin, Yves Cambet, Christèle Frémaux, and Alexander Scheer

2005

Screening factors effecting a response in soluble protein expression
Analytical Biochemistry
Volume: 351. Issue: . Pages: 122–127. Year: 2005. Susanne E. Swalley, John R. Fulghum, Stephen P. Chambers

Agonist-induced calcium response in single human platelets assayed in a micro fluidic device
Analytical Biochemistry
Volume: 341. Issue: . Pages: 361-368. Year: 2005. Louie Tran, Javier Farinas, Lily Ruslim-Litrus, Pamela B. Conley, Craig Muir, Kevin Munnelly, David M. Sedlock, Diana B. Cherbavaz

2004

Comparison of On-Chip and Off-Chip Microfluidic Kinase Assay Formats
ASSAY and Drug Development Technologies
Volume: 2. Issue: 2. Pages: 121-129. Year: 2004. Jude Dunne, Holly Reardon, Vy Trinh, Ella Li, and Javier Farinas

2002

Separation of phospholipids in microfluidic chip device
Analytical Biochemistry
Volume: 314. Issue: . Pages: 97-107. Year: 2002. Sansan Lin, Anthony S. Fischl, Xiahui Bi, and Wally Parce