Systems & Software

Fluorescent Imaging    3D Imaging

Fluorescent Imaging

Fluorescent images for the ventral side of a control mouse (Nu/nu male), illustrating animal tissue autofluorescence.



Bioluminescent (left) and fluorescent (right) images for a subcutaneous injection of three million PC3M-luc/DsRed cells in an 11-week-old male Nu/nu mouse.

Publication: Molecular Imaging. Vol. 3, No. 1, January 2004, pp. 9 – 23

Bioluminescent (left) and fluorescent (right) images for an intramuscular injection of one million HeLa-luc/PKH26 cells in an 11-week-old male Nu/nu mouse.

Publication: Molecular Imaging. Vol. 3, No. 1, January 2004, pp. 9 – 23

Autofluorescent background correction data using the filter subtraction technique. The 6-week-old female Nu/nu mouse was injected subcutaneously with one million HeLa-luc/PKH26 cells in the left flank.

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3D Imaging

Presentation: 4th SMI Conference, September, 2005; Cologne, Germany

An in vivo animal experiment was conducted using two luminescent beads: one implanted in the scruff on the dorsal side and the other in the abdominal fat from the ventral side. Spectral data was taken at 560, 580, 600 and 620 nm. The DLIT code was unable to reconstruct the bead in the abdominal fat for the dorsal data or in the scruff for the ventral data. (λ = 560, 580, 600 and 620 nm)

Presentation: 4th SMI Conference, September, 2005; Cologne, Germany

A Nu/nu mouse was injected with 2x10⁶ PC3M-luc cells in 100 L of PBS into the left ventricle of the heart. The cells were allowed to colonize and grow for 29 days before imaging. Metastases were found on the exterior rib cage, the peritoneal cavity and the left femur. An example of the value of DLIT is demonstrated by the dorsal view data. Even though the average radiance on the surface of the animal for the peritoneal cavity and the left femur are the same, the DLIT reconstruction shows that the peritoneal metastasis is ~50x brighter. (29 days after i.c. injection of 2x10⁶ cells; λ = 580, 600 and 620 nm)




800nm QDs remain in liver and lymph nodes, 800PEG QD never accumulate in liver and lymph nodes. Signal decreases with time due to the QDs being cleared from the body.

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